http://hdl.handle.net/1721.1/18185 http://hdl.handle.net/1721.1/49523 BBF RFC 16: Synthetic Biology Open Language Visual (SBOLv) Specification Rodriguez, Cesar Bartram, Suzie Ramasubramanian, Anusuya Endy, Drew Synthetic Biology Open Language Visual (SBOLv) is a graphical notation that supports biological device development. It provides a formal notation for describing the physical composition of basic parts into composite parts during the development of biological devices. It is targeted for use by biological engineers in forward engineering projects. It encourages and supports model-driven engineering. http://hdl.handle.net/1721.1/49522 The USER cloning standard Kaas, Christian Schrøder Hansen, Niels Bjørn Genee, Hans Jasper Olsen, Lars Rønn Matos, Cláudia Bonde, Mads Tvillinggaard Hansen, Bjarne Gram This BioBricks Foundation Request for Comments (BBF RFC) provides information about the design of uracil-containing primers used for USER cloning and USER fusion. http://hdl.handle.net/1721.1/49521 Large-Scale Peptide Modification on BioBrick Proteins Tian, Feng Chen, GuoQiang Wang, Zhao With the established BioBrick Assembly standards, fusion proteins with diversified functions are easy to manipulate. However, we realized that the existing BBF RFC do not clearly defining the standard for peptide display on BioBrick proteins. Considering the promising use of specific small peptide modification on functional proteins, targeting to specific cell types, for example, we proposed a standard for small peptide modification on BioBrick proteins. http://hdl.handle.net/1721.1/49520 Design of Specific Mammalian Promoters by in silico Prediction Heinemann, Tim Kramer, Stephen Velten, Lars Kranz, Anna-Lena Bauer, Tobias Faura, Marti Bernardo Konig, Rainer Keienburg, Jens Eils, Roland Iwamoto, Nao The purpose of this RFC is to provide a) a method for the design of rational synthetic promoter sequences based on a statistical analysis about the spatial preference of transcription factor binding sites in human promoter sequences and b) further introduce standards to provide compatibility with data formats introduced in this RFC. Description of promoters generated by this method can be found at http://2009.igem.org/Team:Heidelberg/HEARTBEAT_database.