http://hdl.handle.net/1721.1/18186 Sun, 19 May 2013 18:25:55 GMT 2013-05-19T18:25:55Z http://hdl.handle.net/1721.1/78009 Integrases, Aliens, & Bill Joy Endy, Drew Research presentation on recent and unpublished work before the MIT Synthetic Biology Working Group on 24 September 2012 in MIT Building 56. synthetic biology, engineering genetic data storage, logic, and communication systems using phage and integrases Wed, 27 Mar 2013 04:00:00 GMT http://hdl.handle.net/1721.1/78009 2013-03-27T04:00:00Z http://hdl.handle.net/1721.1/65066 Assembly of BioBrick standard biological parts using three antibiotic assembly Shetty, Reshma; Lizarazo, Meagan; Rettberg, Randy; Knight, Thomas F., Jr. An underlying goal of synthetic biology is to make the process of engineering biological systems easier and more reliable. In support of this goal, we developed BioBrick assembly standard 10 to enable the construction of systems from standardized genetic parts. The BioBrick standard underpins the distributed efforts by the synthetic biology research community to develop a collection of more than 6000 standard genetic parts available from the Registry of Standard Biological Parts. Here, we describe the three antibiotic assembly method for physical composition of BioBrick parts and provide step-by-step protocols. The method relies on a combination of positive and negative selection to eliminate time- and labor-intensive steps such as column cleanup and agarose gel purification of DNA during part assembly. This is a revised personal version of the text of the final journal article available via DOI: 10.1016/B978-0-12-385120-8.00013-9 Fri, 20 May 2011 04:00:00 GMT http://hdl.handle.net/1721.1/65066 2011-05-20T04:00:00Z http://hdl.handle.net/1721.1/50999 The BioBrick Public Agreement v1a Endy, Drew; Grewal, David; Schultz, Jason; Lynch, Jennifer; Crews, Lee; Fischer, Mark We present an updated, clean draft for additional public comment of a new legal framework supporting the future of biotechnology. The framework, named here as the BioBrick Public Agreement, is based upon the contribution of promises not to assert any property rights against users of so-contributed standard biological parts. Comments and feedback on this working draft are requested. This draft, BPA v1a, replaces and earlier draft, BPA v1. Mon, 25 Jan 2010 16:29:55 GMT http://hdl.handle.net/1721.1/50999 2010-01-25T16:29:55Z http://hdl.handle.net/1721.1/49434 The BioBrick Public Agreement v1 (draft) Fischer, Mark; Crews, Lee; Lynch, Jennifer; Schultz, Jason; Grewal, David; Endy, Drew We present a draft for public comment of a new legal framework supporting the future of biotechnology. The framework, named here as the BioBrick Public Agreement, is based upon the contribution of promises not to assert any property rights against users of so-contributed standard biological parts. Comments and feedback on this working draft are requested. Sun, 18 Oct 2009 14:13:45 GMT http://hdl.handle.net/1721.1/49434 2009-10-18T14:13:45Z