http://hdl.handle.net/1721.1/7790 Wed, 19 Jun 2013 22:37:13 GMT 2013-06-19T22:37:13Z http://hdl.handle.net/1721.1/79323 Heterogeneity in the population response of a human cell line to hydrogen peroxide as measured by a genetically encoded sensor Ali, Sohail Feroz Genetically encoded ratiometric sensors can provide valuable mechanistic understanding of biological systems. Characterization of cellular response of these sensors is the first step in validating their use. Here, we characterize the response of a genetically encoded H₂O₂ sensor, HyPer, expressed in HeLa cells. Using quantitative fluorescence microscopy, we found significant heterogeneity in HyPer response among the cell population. Further analysis showed that the variation in HyPer response was dependent on expression of HyPer protein as well as on cell cycle phase. Cells with higher levels of expressed HyPer protein showed a stronger HyPer response to H₂O₂. Cells synchronized in S-phase showed a weaker HyPer response than unsynchronized cells. It was determined that this weaker response could be a function of higher antioxidant capacity in S-phase cells. The dependence of HyPer response on these factors needs to be accounted for to avoid experimental artifacts. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2013.; Cataloged from PDF version of thesis.; Includes bibliographical references (p. 22-26). Tue, 01 Jan 2013 00:00:00 GMT http://hdl.handle.net/1721.1/79323 2013-01-01T00:00:00Z http://hdl.handle.net/1721.1/77761 Absorption coefficients in light oil scrubbers Forbes, Edward Colin; Kao, John Yü-Ling Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1941.; Includes bibliographical references (leaf 91). Wed, 01 Jan 1941 00:00:00 GMT http://hdl.handle.net/1721.1/77761 1941-01-01T00:00:00Z http://hdl.handle.net/1721.1/76489 Mergers and acquisitions process improvement in a matrix organization Cavazos Cavazos, Jose Juan 3M has had aggressive growth through acquisitions. Almost all of the acquisitions add new manufacturing sites to the existing manufacturing and distribution networks. 3M is composed of 38 divisions (as of 2011) with significant independence. There are corporate experts in the different functional areas of due diligence that bring consistency regardless of the division doing the acquisition. However, since manufacturing is not centralized and is managed by each division, there is no corporate expert neither for manufacturing due diligence or supply chain. The difference in the experience level of manufacturing managers in different divisions, and a division-centric view of the supply chain has lead sometimes to integration plans that don't fully take into account opportunities in supply chain rationalization and future manufacturing improvements. The goal of this study is to map the current process and tools across divisions, to identify gaps and to create a business process that can be used as a guideline for all the divisions engaging in acquisitions with a manufacturing component. In addition, a principal components analysis is proposed to find which parameters could predict the outcome of an acquisition. Thesis (M.B.A.)--Massachusetts Institute of Technology, Sloan School of Management; and, (S.M.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering; in conjunction with the Leaders for Global Operations Program at MIT, 2012.; Cataloged from PDF version of thesis.; Includes bibliographical references (p. 70). Sun, 01 Jan 2012 00:00:00 GMT http://hdl.handle.net/1721.1/76489 2012-01-01T00:00:00Z http://hdl.handle.net/1721.1/74849 Mixing in a fluid flowing through a packed bed Danckwerts, P. V., 1916-; Sugden, A. C Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1948.; Includes bibliographical references (leaf 68). Thu, 01 Jan 1948 00:00:00 GMT http://hdl.handle.net/1721.1/74849 1948-01-01T00:00:00Z