Antibiotics induce polarization of pleural macrophages to M2-like phenotype in patients with tuberculous pleuritis
Author(s)
Wang, Sisi; Zhang, Jian; Sui, Liyan; Xu, Hao; Piao, Qianling; Qu, Xinglong; Sun, Ying; Song, Lei; Peng, Liping; Hua, Shucheng; Hu, Guangan; Chen, Jianzhu; Li, Dan, 1969-; Liu, Ying, Ph. D. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; ... Show more Show less
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Pleural macrophages play critical roles in pathogenesis of tuberculous pleuritis, but very little is known about their response to anti-tuberculosis antibiotics treatment. Here, we examined whether and how pleural macrophages change in phenotype, transcription and function following antibiotics treatment in patients with tuberculous pleuritis. Results show pro-inflammatory cytokines were down-regulated significantly post antibiotic treatment in the pleural effusions and pleural macrophages up-regulated markers characteristic of M2 macrophages such as CD163 and CD206. Differential expression analysis of transcriptomes from four paired samples before and after treatment identified 230 treatment-specific responsive genes in pleural macrophages. Functional analysis identified interferon-related pathway to be the most responsive genes and further confirmed macrophage polarization to M2-like phenotype. We further demonstrate that expression of a significant fraction of responsive genes was modulated directly by antibiotics in pleural macrophages in vitro. Our results conclude that pleural macrophages polarize from M1-like to M2-like phenotype within a mean of 3.5 days post antibiotics treatment, which is dependent on both pleural cytokine environment and direct modulatory effects of antibiotics. The treatment-specific genes could be used to study the roles of pleural macrophages in the pathogenesis of tuberculous pleuritis and to monitor the response to antibiotics treatment.
Date issued
2017-11Department
Koch Institute for Integrative Cancer Research at MITJournal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Wang, Sisi et al. “Antibiotics Induce Polarization of Pleural Macrophages to M2-Like Phenotype in Patients with Tuberculous Pleuritis.” Scientific Reports 7, 1 (November 2017): 14982 © 2017 The Author(s)
Version: Final published version
ISSN
2045-2322