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Functions of alternative ClpP subunits in Pseudomonas aeruginosa
(Massachusetts Institute of Technology, 2020)
Proteolysis is the process by which proteins are broken down, or hydrolyzed, into small peptides or amino acids by enzymes. Cells from all forms of life carry out regulated protein degradation as a way to control cellular ...
Characterization of synaptotagmin 7 function in neurotransmission and its subcellular localization at synapses
(Massachusetts Institute of Technology, 2020)
Synaptic vesicle (SV) fusion is dependent on proteins that can sense Ca²⁺ and trigger fusion with the plasma membrane. Neurotransmitter release occurs during a rapid synchronized phase of SV fusion mediated by the Ca²⁺ ...
Insights into gene regulation by genome structure, phase separation and developmental signaling
(Massachusetts Institute of Technology, 2020)
Proper regulation of gene expression is essential to the developmental processes that give rise to hundreds of different cell types with unique cellular identities. Regulation of protein-coding and long non-coding RNA genes ...
Investigation of two-component signaling systems in Pseudomonas aeruginosa and their roles in the mucus barrier
(Massachusetts Institute of Technology, 2021)
All living things must be able to sense and respond to signals in their environment in order to grow and survive. For bacteria, a common means through which this occurs is via two-component signaling systems, which are ...
Understanding microRNA targeting with high-throughput biochemistry
(Massachusetts Institute of Technology, 2021)
MicroRNAs (miRNAs) are short RNAs that, in complex with Argonaute (AGO) proteins, guide repression of mRNA targets. miRNAs negatively regulate most mammalian mRNAs, and disruption of this regulation often results in severe ...
Defining the role of aneuploidy throughout tumorigenesis
(Massachusetts Institute of Technology, 2021)
Aneuploidy is a state of genome imbalance which alters the copy number of whole chromosomes. While aneuploidy is rare in healthy tissues, it is one of the most common features of cancerous tumors. Studies of aneuploid yeast ...
Transcriptome-wide organization of subcellular microenvironments revealed by ATLAS-Seq
(Massachusetts Institute of Technology, 2020)
Subcellular localization of RNAs is a ubiquitous and evolutionarily conserved process that provides an additional layer of transcriptome organization promoting coordinated control of gene expression in both space and time. ...
Genome organization in transcriptional regulation
(Massachusetts Institute of Technology, 2021)
Transcriptional regulation of gene expression plays critical roles in the control of cell identity, development, and disease. Genome organization contributes to transcriptional regulation in multiple ways. At a fundamental ...
Intersubunit communication and coordinated mechanical activity in the AAA+ protease ClpXP
(Massachusetts Institute of Technology, 2020)
Proteases belonging to the AAA+ (ATPases associated with various cellular activities) family perform regulated proteolysis in all domains of life by binding, mechanically unfolding, and degrading target proteins. The ...
Single-molecule studies of the mechanism of eukaryotic helicase activation
(Massachusetts Institute of Technology, 2021)
Eukaryotic DNA replication is a fundamental process that must occur accurately and only once per cell cycle. To ensure that origins only initiate once per cell cycle, the events of DNA replication initiation are temporally ...