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The roles of the helicase double-hexamer complex and the ssDNA-binding protein RPA during eukaryotic DNA replication
(Massachusetts Institute of Technology, 2020)
Eukaryotic DNA replication is a complex process that must occur accurately, completely, and only once per cell cycle. To accomplish these goals, the events of DNA replication are tightly coupled to cell-cycle progression. ...
Effects of the mobile genetic element ICEBs1 on bacterial host fitness
(Massachusetts Institute of Technology, 2020)
Mobile genetic elements drive bacterial evolution by mediating horizontal gene transfer and by carrying cargo genes that confer important traits to host cells. Traits provided by mobile genetic elements include antibiotic ...
Understanding the mechanism of antiretroviral nucleoside analogs as inhibitors of Epstein-Barr virus lytic DNA replication
(Massachusetts Institute of Technology, 2020)
Epstein-Barr virus (EBV) is a human B-cell tropic double-stranded DNA y-herpesvirus. To date, there are no antiviral agents proven to be clinically effective in the treatment of EBV infection or EBV-associated diseases. ...
Molecular, cellular, and circuit analysis of C. elegans spitting behavior
(Massachusetts Institute of Technology, 2020)
To identify the neural and molecular substrates of animal behavior and understand the principles by which they function is a major goal in neurobiology. One simple system for such behavioral studies is the pharynx of the ...
Mapping the therapy resistance landscapes of acute leukemias using in vivo functional genomics
(Massachusetts Institute of Technology, 2020)
The recurrence of therapy resistant disease remains an intractable problem in oncology clinical care. To address this issue, investigators have traditionally focused on elucidating cell-intrinsic mechanisms that render ...
Reverse genetic approaches reveal gene redundancy in Arabidopsis anthers
(Massachusetts Institute of Technology, 2020)
When aquatic plants migrated to land 500 million years ago, they were met with harsh conditions associated with terrestrial life, such as dry air, high radiance light, and high effective gravity. Early plants of this time ...
Signal transduction in human cells by metabolites derived from methionine and glucose
(Massachusetts Institute of Technology, 2021)
Organisms sense nutrients to match physiological responses to their environment. The mechanistic Target of Rapamycin complex I (mTORC1) pathway integrates information from a wide range of nutrient inputs to appropriately ...
Tool development for the rapid identification of microbiome manipulating agents
(Massachusetts Institute of Technology, 2020)
Manipulation of complex microbial communities, such as human microbiomes, plays a critical role in the study and treatment of microbiome associated diseases. However, the tools available to perform microbiome manipulations ...
Activin signaling controls a wound-induced program essential for regenerative patterning
(Massachusetts Institute of Technology, 2020)
A central problem in animal regeneration is how animals determine what body part to regenerate. Planarians are flatworms that can regenerate any missing body part, and are studied to identify mechanisms underlying regeneration. ...
Form and function of Poly(A) tails
(Massachusetts Institute of Technology, 2020)
Central to mRNA metabolism is the poly(A)-tail, a stretch of adenosine nucleotides at the mRNA 3' end. In this dissertation, I investigate the role of the tail in the dynamics of mRNA decay, and describe the predominant ...