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Browsing Synthetic Biology by Title

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  • Design of Specific Mammalian Promoters by in silico Prediction 
    Heinemann, Tim; Kramer, Stephen; Velten, Lars; Kranz, Anna-Lena; Bauer, Tobias; Faura, Marti Bernardo; Konig, Rainer; Keienburg, Jens; Eils, Roland; Iwamoto, Nao (2009-10-30)
    The purpose of this RFC is to provide a) a method for the design of rational synthetic promoter sequences based on a statistical analysis about the spatial preference of transcription factor binding sites in human promoter ...
  • Detailed Information Standard 
    Culviner, Peter; Pantalone, Nathaniel; Sagstetter, Mary; Sandock, Sarah; Vrana, Justin; Wu, Yue (2010-12-05)
    The purpose of this standard is to increase the required information that must accompany any parts submitted to the Registry of Standard Biological Parts. This will give users of the parts better assurance of their ...
  • Documentation of BioBrick parts 
    Slomp, Arend; Ekkers, David (2010-12-05)
    Purpose of this RFC is to improve the information supplied by BioBrick part vendors. Reason to have this RFC is that there would be much more information available which can help improve the search time of the user. ...
  • Draft Standard for Biobrick BB-2 Biological Parts 
    Knight, Tom (OpenWetWare, 2008-11-19)
    This standard defines the required sequence properties for a Biobrick(R) BB-2 standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards.
  • Draft Standard for Biobrick Biological Parts 
    Knight, Tom (OpenWetWare, 2007-05-03)
    This standard defines the required sequence properties for a Biobrick(tm) standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards. ...
  • DTU Synthetic Promoter Library Standard 
    Fortuna, Patrick; Trolle, Thomas; Borch, Martin Malthe; Haugaard, Anastasiya; Kjaergaard, Maya Friis; Iversen, Lisa Blanc; Jollmann, Annemi; Sander, Anja; Frederiksen, Juliet; Slodkowicz, Grzegorz (2010-12-04)
    The purpose of this RFC is to outline a method for generating a BioBrick compatible Synthetic Promoter Library (SPL) within bacteria in order to fine-tune the expression of BioBrick parts and devices.
  • Engineered Post-Translational Logic (PTL) 
    Sutton, Samantha; Neves, Sara; Leung, Lauren; Endy, Drew (2005-12-07)
    Current synthetic biological circuits make use of protein-DNA and RNA-RNA interactions to control gene expression in bacteria. Systems that rely on the regulation of gene expression are relatively slow and unsuitable for ...
  • Engineering the Interface Between Cellular Chassis and Integrated Biological Systems 
    Canton, Bartholomew (2005-08-08)
    The engineering of biological systems with predictable behavior is a challenging problem. One reason for this difficulty is that engineered biological systems are embedded within complex and variable host cells. To help ...
  • Engineering the Interface Between Cellular Chassis and Integrated Biological Systems 
    Canton, Bartholomew; Endy, Drew (2005-10-21)
    The engineering of biological systems with predictable behavior is a challenging problem. One reason for this difficulty is that engineered biological systems are embedded within complex and variable host cells. To help ...
  • Engineering transcription-based digital logic devices 
    Shetty, Reshma P.; Endy, Drew; Knight, Thomas F. Jr (2007-07-17)
    Implementing in vivo information processing is a key challenge in synthetic biology. We describe the construction and characterization of digital transcription-based devices from zinc fingers and leucine zippers. We also ...
  • Engineering transcription-based digital logic devices 
    Shetty, Reshma P.; Knight, Thomas F. Jr (Sixth International Conference on Systems Biology, 2005-10-20)
    The goal of Synthetic Biology is to engineer systems from biological parts. One class of systems are those whose purpose is to process information. My work seeks to build transcription-based devices for use in combinational ...
  • Fast multiple gene fragment ligation method based on homologous recombination 
    Wang, Ruiyan; Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
    With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at ...
  • Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII 
    Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
    With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at ...
  • Fluorescence assay for polymerase arrival rates 
    Che, Austin, 1979- (Massachusetts Institute of Technology, 2004)
    To engineer complex synthetic biological systems will require modular design, assembly, and characterization strategies. The RNA polymerase arrival rate (PAR) is defined to be the rate that RNA polymerases arrive at a ...
  • Fluorescence Assay for Polymerase Arrival Rates 
    Che, Austin (2003-08-31)
    To engineer complex synthetic biological systems will require modular design, assembly, and characterization strategies. The RNA polymerase arrival rate (PAR) is defined to be the rate that RNA polymerases arrive at a ...
  • From Genetically Modified Organisms To Synthetic Biology: Legislation in the European Union, in Six Member Countries and in Switzerland 
    Furger, Franco (2007-12-04)
    This report is based on the assumption that in Europe and in its member countries—with the exception perhaps of Switzerland—“synthetic genomics” as a distinct policy domain does not yet exist. This conclusion is based ...
  • Fusion Protein (Freiburg) Biobrick assembly standard 
    Grünberg, Raik; Arndt, Katja; Müller, Kristian (2009-04-18)
    This Request for Comments (RFC) describes an extension to the original BioBrick assembly standard (BBF RFC 10). The Fusion Assembly strategy described here is fully backwards compatible with RFC 10 and supports the ...
  • GeneJax: A Prototype CAD tool in support of Genome Refactoring 
    Anand, Ishan; Kosuri, Sriram; Endy, Drew (2006-05-26)
    Refactoring is a technique used by computer scientists for improving program design. The Endy Laboratory has adapted this process to make the genomes of biological organisms more amenable to human understanding and design ...
  • General updating scheme for the design and construction of assembly vectors that are compatible with BBF RFC 10 and BBF RFC 28 
    Billerbeck, Sonja; Zwicky, Katharina; Lang, Moritz; Pestalozzi, Luzius; Rosenberger, George; Constantinescu, Simona; Ambegoda, Thanuja; Sotiriadis, Elsa (2010-12-05)
    BBF RFC 75 intends to link the BBF RFC 28-based combinatorial multi-part assembly method to BBF RFC 10. Its aim is to easily redesign an expression vector to make it compatible to BBF RFC 10 for the construction of fusion ...
  • The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly 
    Pauthenier, Cyrille; El-Sayyed, Hafez; Rostain, William; Cerisy, Tristan; Lopez, Carolina Gallo; Ferreira, Raphael; Moreira, Jorgelindo Da Veiga; Souterre, Tiffany; Eeckhout, Joachim; Nogue, Pierre Yves; Llamosi, Artemis; Parutto, Pierre; Nikolayeva, Iryna; Machat, Mohamed; Marquet, Clement; Młynarczyk, Anna; Thelie, Aurore; Landrain, Thomas; Tolonen, Andrew; Pollet, Nicolas; Faulon, Jean-Loup; Jaramilo, Alfonso (2012-11-05)
    BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ...
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