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Browsing Synthetic Biology by Title

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  • Fast multiple gene fragment ligation method based on homologous recombination 
    Wang, Ruiyan; Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
    With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at ...
  • Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII 
    Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
    With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at ...
  • Fluorescence Assay for Polymerase Arrival Rates 
    Che, Austin (2003-08-31)
    To engineer complex synthetic biological systems will require modular design, assembly, and characterization strategies. The RNA polymerase arrival rate (PAR) is defined to be the rate that RNA polymerases arrive at a ...
  • Fluorescence assay for polymerase arrival rates 
    Che, Austin, 1979- (Massachusetts Institute of Technology, 2004)
    To engineer complex synthetic biological systems will require modular design, assembly, and characterization strategies. The RNA polymerase arrival rate (PAR) is defined to be the rate that RNA polymerases arrive at a ...
  • From Genetically Modified Organisms To Synthetic Biology: Legislation in the European Union, in Six Member Countries and in Switzerland 
    Furger, Franco (2007-12-04)
    This report is based on the assumption that in Europe and in its member countries—with the exception perhaps of Switzerland—“synthetic genomics” as a distinct policy domain does not yet exist. This conclusion is based ...
  • Fusion Protein (Freiburg) Biobrick assembly standard 
    Grünberg, Raik; Arndt, Katja; Müller, Kristian (2009-04-18)
    This Request for Comments (RFC) describes an extension to the original BioBrick assembly standard (BBF RFC 10). The Fusion Assembly strategy described here is fully backwards compatible with RFC 10 and supports the ...
  • GenBrick – A Rapid Multi-Part Assembly Method for BioBricks 
    Gasiūnaitė, Lina; Lewicka, Aleksandra; Pashkuleva, Hristiana; Villanueva, Hugo; Thornton, Harry; Trubitsyna, Maryia; French, Chris (2013-10-04)
    This BioBricks Foundation Request for Comments (BBF RFC) describes a novel approach, GenBrick, for the rapid assembly of multiple BioBrick RFC10-compatible parts in a single reaction, with completely flexible part order, ...
  • GeneJax: A Prototype CAD tool in support of Genome Refactoring 
    Anand, Ishan; Kosuri, Sriram; Endy, Drew (2006-05-26)
    Refactoring is a technique used by computer scientists for improving program design. The Endy Laboratory has adapted this process to make the genomes of biological organisms more amenable to human understanding and design ...
  • General updating scheme for the design and construction of assembly vectors that are compatible with BBF RFC 10 and BBF RFC 28 
    Billerbeck, Sonja; Zwicky, Katharina; Lang, Moritz; Pestalozzi, Luzius; Rosenberger, George; Constantinescu, Simona; Ambegoda, Thanuja; Sotiriadis, Elsa (2010-12-05)
    BBF RFC 75 intends to link the BBF RFC 28-based combinatorial multi-part assembly method to BBF RFC 10. Its aim is to easily redesign an expression vector to make it compatible to BBF RFC 10 for the construction of fusion ...
  • The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly 
    Pauthenier, Cyrille; El-Sayyed, Hafez; Rostain, William; Cerisy, Tristan; Lopez, Carolina Gallo; Ferreira, Raphael; Moreira, Jorgelindo Da Veiga; Souterre, Tiffany; Eeckhout, Joachim; Nogue, Pierre Yves; Llamosi, Artemis; Parutto, Pierre; Nikolayeva, Iryna; Machat, Mohamed; Marquet, Clement; Młynarczyk, Anna; Thelie, Aurore; Landrain, Thomas; Tolonen, Andrew; Pollet, Nicolas; Faulon, Jean-Loup; Jaramilo, Alfonso (2012-11-05)
    BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ...
  • HiCT: High Throughput Protocols For CPE Cloning And Transformation 
    Beer, Ralf; Christiansen, Tania; Herbst, Konrad; Ignatiadis, Nikolaos; Kats, Ilia; Kurzawa, Nils; Meichsner, Johanna; Rabe, Sophie; Riedel, Anja; Sachs, Joshua; Schessner, Julia; Schmidt, Florian; Walch, Philipp; Adlung, Lorenz; Genereith, Katharina; Georgi, Fanny; Heinemann, Tim; Meyer, Hannah; Niopek, Dominik; Di Ventura, Barbara; Eils, Roland (2013-10-04)
    The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...
  • How to Build Kinetic Models of BioBricks 
    Weeding, Emma; Houle, Jason; Swiniarski, Ben; Smadbeck, Patrick; Lindblad, Kristen; Volzing, Katherine; Srivastava, Poonam; Sotiropoulos, Vassilios; Biliouris, Kostas; Kaznessis, Yiannis (2009-10-21)
    This BioBricks Foundation Request for Comments (BBF RFC) provides instructions on how to automatically generate a model of any BioBrick sequence. These models can be used in computer simulations of the dynamic behavior of ...
  • Idempotent Vector Design for Standard Assembly of Biobricks 
    Knight, Thomas (MIT Artificial Intelligence Laboratory; MIT Synthetic Biology Working Group, 2003)
    The lack of standardization in assembly techniques for DNA sequences forces each DNA assembly reaction to be both an experimental tool for addressing the current research topic, and an experiment in and of itself. One ...
  • iGEM 2005 Invitation Supplement 
    Endy, Drew (2005-09-29)
    This document provides background material in support of the 2005 intercollegiate Genetically Engineered Machine Competition (aka iGEM). Herein, you can read quick summaries of earlier competitions and courses, obtain a ...
  • Impact of Synthetic Genomics on the Threat of Bioterrorism with Viral Agents 
    Collett, Marc (2007-12-04)
    In 2002, a team of researchers at the State University of New York led by Eckard Wimmer assembled a DNA template for the RNA poliovirus using an internet-available nucleotide sequence and mail order synthetic ...
  • The Imperative of Synthetic Biology: A Proposed National Research Initiative 
    Lazowska, Ed; Endy, Drew (2008-12-23)
  • Instructions to BBF RFC Authors 
    Tarjan, Daniel; Sleight, Sean; Shetty, Reshma; Ridgway, Doug; Rettberg, Randy; McArthur, George; Mayer, Antonia; Knight, Tom; French, Chris; Endy, Drew; de Mora, Kim; Elfick, Alistair; Cowell, Mackenzie; Che, Austin; Anderson, Chris (OpenWetWare, 2008-11-10)
    This BioBricks Foundation Request for Comments (BBF RFC) provides in- formation about the preparation and submission of BBF RFCs to The Bio- Bricks Foundation (BBF).
  • Integrases, Aliens, & Bill Joy 
    Endy, Drew (2013-03-27)
    Research presentation on recent and unpublished work before the MIT Synthetic Biology Working Group on 24 September 2012 in MIT Building 56.
  • In the valley of the shadow of death 
    Brent, Roger (2006-11-22)
    During the 20th century, advances in biological understanding sparked a global revolution in biological capability, or RBC. Since that time, the revolution has proceeded in a Moore's-law-like fashion for many decades. ...
  • LabView VIs for operating Sortostat 
    Kelly, Jason (2008-05-14)
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