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Browsing Synthetic Biology by Title

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  • Integrases, Aliens, & Bill Joy 
    Endy, Drew (2013-03-27)
    Research presentation on recent and unpublished work before the MIT Synthetic Biology Working Group on 24 September 2012 in MIT Building 56.
  • In the valley of the shadow of death 
    Brent, Roger (2006-11-22)
    During the 20th century, advances in biological understanding sparked a global revolution in biological capability, or RBC. Since that time, the revolution has proceeded in a Moore's-law-like fashion for many decades. ...
  • LabView VIs for operating Sortostat 
    Kelly, Jason (2008-05-14)
  • Large-Scale Peptide Modification on BioBrick Proteins 
    Tian, Feng; Chen, GuoQiang; Wang, Zhao (2009-10-30)
    With the established BioBrick Assembly standards, fusion proteins with diversified functions are easy to manipulate. However, we realized that the existing BBF RFC do not clearly defining the standard for peptide display ...
  • May 2006 correspondance between James Randerson and Drew Endy 
    Endy, Drew (2006-06-15)
    I am a science journalist with the Guardian newspaper in London. We are a national daily paper. I am hoping to put together an investigative piece on how easy (or not) it would be for a would-be terrorist to put together ...
  • Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR 
    Olchowik, Anna; Lower, Michal; Moreno, Cherry; Pankowski, Jaroslaw (2010-12-05)
    The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, ...
  • The Measurement of rho‐independent Transcription Terminator Efficiency 
    He, Jiankui; Jing, Shui; Zhang, Mengshi; Liu, Xianggeng; Chen, Yao; Qie, Boyu; Shen, Liuxing; Wu, Zishan (2012-10-11)
    The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of terminators in bacteria. Because we have characterized the performance of terminator in E. coli and used a simple ...
  • miMeasure – a standard for miRNA binding site characterization in mammalian cells 
    Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; Flocke, Lea; Kleinsorg, Stefan; Kolodziejczyk, Aleksandra; Kraemer, Stephen; Torre, Alejandro Macias; Mathur, Aastha; Mayilo, Dmytro; Neumann, Stefan; Niopek, Dominik; Pisa, Rudolf; Schad, Jan-Ulrich; Schumacher, Laura-Nadine; Uhlig, Thomas; Wu, Xiaoting; Keienburg, Jens; Boerner, Kathleen; Grimm, Dirk; Eils, Roland (2010-12-05)
    This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...
  • The minimum information for a qualified BioBrick 
    Zhou, Mubing; Zhang, Junqiu; He, Jiankui (2012-10-11)
    Since the information of many existing BioBricks is incomplete, thus the usage of the BioBricks will be affected. It is necessary to standardize the minimum information required for a qualified BioBrick. Furthermore this ...
  • miTuner - a kit for microRNA based gene expression tuning in mammalian cells 
    Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; Flocke, Lea; Kleinsorg, Stefan; Kolodziejczyk, Aleksandra; Kraemer, Stephen; Torre, Alejandro Macias; Mathur, Aastha; Mayilo, Dmytro; Neumann, Stefan; Niopek, Dominik; Pisa, Rudolf; Schad, Jan-Ulrich; Schumacher, Laura-Nadine; Uhlig, Thomas; Wu, Xiaoting; Keienburg, Jens; Boerner, Kathleen; Grimm, Dirk; Eils, Roland (2010-12-05)
    The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ...
  • MONOD, a Collaborative Tool for Manipulating Biological Knowledge 
    Soergel, David; Choi, Kirindi; Thomson, Ty; Doane, Jay; George, Brian; Morgan-Linial, Ross; Brent, Roger; Endy, Drew (2008-06-19)
    We describe an open source software tool called MONOD, for Modeler’s Notebook and Datastore, designed to capture and communicate knowledge generated during the process of building models of many-component biological ...
  • Naming of standards of physical composition of BioBrick parts 
    Rettberg, Randy; Shetty, Reshma (2009-04-17)
    This BioBricks Foundation Request for Comments (BBF RFC) proposes a naming convention for standards of physical composition of BioBrick parts in which assembly standards are named according to the BBF RFC that specifies ...
  • A New Biobrick Assembly Strategy Designed for Facile Protein Engineering 
    Phillips, Ira; Silver, Pamela (2006-04-20)
    The existing biobrick assembly technique[1] provides a straightforward way to combine standardized biological components, termed biobricks. This system, however, is limited in that each protein-encoding biobrick must ...
  • A New Standard to Connect BioBrick Parts for Precise Extraction of an Enzyme Digestion Product 
    Uekusa, Kousuke; Iguchi, Seiko (2010-12-05)
    This BioBricks Foundation Request for Comments (BBF RFC) introduces a new standard to connect BioBrick parts using BglI site.
  • Novel Normalization Standard using Fluorescence 
    Bukhari, Syed Habib Tahir; Akkineni, Ashwini Rahul; Ananth, Adithya; Gordeev, Victor; Grieb, Svea; Mansour, Sarah; Roth, Mareike; Sampathkumar, Charanya; Schirmer, Lucas; Tam, Jonathan (2010-12-05)
    The Biotec_Dresden Team 2010 developed an approach where two fluorescent proteins are simultaneously expressed. The fact that one reporter, in our case RFP, is constitutively expressed allows to monitor cell growth. Secondly, ...
  • Open licensing of BioBrick parts 
    Lower, Michal; Olchowik, Anna; Pankowski, Jaroslaw; Bazlekowa, Milena; Blaszkiewicz, Marta; Cysewski, Dominik; Koper, Kamil; Lesczynska, Joanna; Moreno, Cherry; Pulawska, Anna (2010-12-05)
    This document provides recommendations for licensing of community-created biological parts, especially in BioBrick standard.
  • pCH1497-ASD1 
    Canton, Bartholomew (2008-05-23)
  • PCR - Ligation Assembly Standard for BioBrick Parts 
    He, Tony PeiYuan; Campbell, Alexander; Zhou, Stefanie Yifan (2011-12-15)
    This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This ...
  • Power and responsibility 
    Brent, Roger (2006-11-21)
    Peter Parker and Uncle Ben are on my mind. The reason is that is that a month ago I was jumped by Craig Venter. There were TV cameras around. The live audience was an interesting, edgy mix, on the interface between ...
  • A Practical Perspective on DNA Synthesis and Biological Security (12/4/2006 Draft) 
    Bügl, Hans; Danner, John; Molinari, Robert; Mulligan, John; Roth, David; Wagner, Ralf; Budowle, Bruce; Scripp, Robert; Smith, Jenifer; Steele, Scott; Church, George; Endy, Drew (2006-12-12)
    Few developments have leapfrogged over predecessor technology as quickly and extensively as synthetic biology. Based on cutting-edge DNA synthesis technology, synthetic biology has already fueled an expansion of ...
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