Browsing Synthetic Biology by Title
Now showing items 87-106 of 139
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LabView VIs for operating Sortostat
(2008-05-14) -
Large-Scale Peptide Modification on BioBrick Proteins
(2009-10-30)With the established BioBrick Assembly standards, fusion proteins with diversified functions are easy to manipulate. However, we realized that the existing BBF RFC do not clearly defining the standard for peptide display ... -
May 2006 correspondance between James Randerson and Drew Endy
(2006-06-15)I am a science journalist with the Guardian newspaper in London. We are a national daily paper. I am hoping to put together an investigative piece on how easy (or not) it would be for a would-be terrorist to put together ... -
Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR
(2010-12-05)The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, ... -
The Measurement of rho‐independent Transcription Terminator Efficiency
(2012-10-11)The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of terminators in bacteria. Because we have characterized the performance of terminator in E. coli and used a simple ... -
miMeasure – a standard for miRNA binding site characterization in mammalian cells
(2010-12-05)This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ... -
The minimum information for a qualified BioBrick
(2012-10-11)Since the information of many existing BioBricks is incomplete, thus the usage of the BioBricks will be affected. It is necessary to standardize the minimum information required for a qualified BioBrick. Furthermore this ... -
miTuner - a kit for microRNA based gene expression tuning in mammalian cells
(2010-12-05)The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ... -
MONOD, a Collaborative Tool for Manipulating Biological Knowledge
(2008-06-19)We describe an open source software tool called MONOD, for Modeler’s Notebook and Datastore, designed to capture and communicate knowledge generated during the process of building models of many-component biological ... -
Naming of standards of physical composition of BioBrick parts
(2009-04-17)This BioBricks Foundation Request for Comments (BBF RFC) proposes a naming convention for standards of physical composition of BioBrick parts in which assembly standards are named according to the BBF RFC that specifies ... -
A New Biobrick Assembly Strategy Designed for Facile Protein Engineering
(2006-04-20)The existing biobrick assembly technique[1] provides a straightforward way to combine standardized biological components, termed biobricks. This system, however, is limited in that each protein-encoding biobrick must ... -
A New Standard to Connect BioBrick Parts for Precise Extraction of an Enzyme Digestion Product
(2010-12-05)This BioBricks Foundation Request for Comments (BBF RFC) introduces a new standard to connect BioBrick parts using BglI site. -
Novel Normalization Standard using Fluorescence
(2010-12-05)The Biotec_Dresden Team 2010 developed an approach where two fluorescent proteins are simultaneously expressed. The fact that one reporter, in our case RFP, is constitutively expressed allows to monitor cell growth. Secondly, ... -
Open licensing of BioBrick parts
(2010-12-05)This document provides recommendations for licensing of community-created biological parts, especially in BioBrick standard. -
Open Sequence Initiative: a part submission standard to complement modern DNA assembly techniques
(2013-10-06)The discipline of synthetic biology emphasizes the application of engineering principles such as standardization, abstraction, modularity, and rational design to complex biological systems. The archetypical example of such ... -
pCH1497-ASD1
(2008-05-23) -
PCR - Ligation Assembly Standard for BioBrick Parts
(2011-12-15)This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This ... -
Power and responsibility
(2006-11-21)Peter Parker and Uncle Ben are on my mind. The reason is that is that a month ago I was jumped by Craig Venter. There were TV cameras around. The live audience was an interesting, edgy mix, on the interface between ... -
A Practical Perspective on DNA Synthesis and Biological Security (12/4/2006 Draft)
(2006-12-12)Few developments have leapfrogged over predecessor technology as quickly and extensively as synthetic biology. Based on cutting-edge DNA synthesis technology, synthetic biology has already fueled an expansion of ... -
Promoter and Coding Sequence Considerations for Caenorhabditis elegans and Other Eukaryotes
(2010-11-07)Our primary aim is to provide a standard format for protein-coding BioBricks intended for eukaryotes, especially in the nematode worm Caenorhabditis elegans, so that future teams will be able to correctly take advantage ...
