Browsing Synthetic Biology by Title
Now showing items 95-114 of 139
-
MONOD, a Collaborative Tool for Manipulating Biological Knowledge
(2008-06-19)We describe an open source software tool called MONOD, for Modeler’s Notebook and Datastore, designed to capture and communicate knowledge generated during the process of building models of many-component biological ... -
Naming of standards of physical composition of BioBrick parts
(2009-04-17)This BioBricks Foundation Request for Comments (BBF RFC) proposes a naming convention for standards of physical composition of BioBrick parts in which assembly standards are named according to the BBF RFC that specifies ... -
A New Biobrick Assembly Strategy Designed for Facile Protein Engineering
(2006-04-20)The existing biobrick assembly technique[1] provides a straightforward way to combine standardized biological components, termed biobricks. This system, however, is limited in that each protein-encoding biobrick must ... -
A New Standard to Connect BioBrick Parts for Precise Extraction of an Enzyme Digestion Product
(2010-12-05)This BioBricks Foundation Request for Comments (BBF RFC) introduces a new standard to connect BioBrick parts using BglI site. -
Novel Normalization Standard using Fluorescence
(2010-12-05)The Biotec_Dresden Team 2010 developed an approach where two fluorescent proteins are simultaneously expressed. The fact that one reporter, in our case RFP, is constitutively expressed allows to monitor cell growth. Secondly, ... -
Open licensing of BioBrick parts
(2010-12-05)This document provides recommendations for licensing of community-created biological parts, especially in BioBrick standard. -
Open Sequence Initiative: a part submission standard to complement modern DNA assembly techniques
(2013-10-06)The discipline of synthetic biology emphasizes the application of engineering principles such as standardization, abstraction, modularity, and rational design to complex biological systems. The archetypical example of such ... -
pCH1497-ASD1
(2008-05-23) -
PCR - Ligation Assembly Standard for BioBrick Parts
(2011-12-15)This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This ... -
Power and responsibility
(2006-11-21)Peter Parker and Uncle Ben are on my mind. The reason is that is that a month ago I was jumped by Craig Venter. There were TV cameras around. The live audience was an interesting, edgy mix, on the interface between ... -
A Practical Perspective on DNA Synthesis and Biological Security (12/4/2006 Draft)
(2006-12-12)Few developments have leapfrogged over predecessor technology as quickly and extensively as synthetic biology. Based on cutting-edge DNA synthesis technology, synthetic biology has already fueled an expansion of ... -
Promoter and Coding Sequence Considerations for Caenorhabditis elegans and Other Eukaryotes
(2010-11-07)Our primary aim is to provide a standard format for protein-coding BioBricks intended for eukaryotes, especially in the nematode worm Caenorhabditis elegans, so that future teams will be able to correctly take advantage ... -
Proposed Conceptual Guidelines for the Design of a BioBrick Graphical Language & an Example
(2009-03-12)As we update the current BioBrick symbols we have an opportunity to look at the end users of a BioBrick Graphical language and develop design guidelines so that the new symbols best suit their needs. This document explores ... -
Provisional BioBrick Language (PoBoL)
(2009-05-15)This BioBricks Foundation Request for Comments (BBF RFC) describes a semantic markup language for publishing and sharing information about BioBricks on the World Wide Web. This BBF RFC includes the recommendation for the ... -
Quantitative measurement of mammalian cell invasion by bacteria using flow cytometry
(2010-10-31)Purpose of this RFC is to provide method of measurement of bacterial invasion into mammalian cells. Also this RFC defines Percent of INvasion (PIN) unit. -
RA-PCR, a method for the generation of randomized promoter libraries
(2009-10-21)The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of ... -
Rapid Characterization of Cellular Pathways Using Time-Varying Signals
(International Conference on Systems Biology, 2005-10-21)The use of traditional tools for the discovery and characterization of biological systems has resulted in a wealth of biological knowledge. Unfortunately, only a small portion of the biological world is well-understood ... -
Raw Data, Win MN, Smolke CD. 2008. Higher-order cellular information processing with synthetic RNA devices. Science. 322: 456-60. DOI: 10.1126/science.1160311
(2009-07-29)This supplement provides additional detail on the data analysis methods and the raw data for the RNA devices presented in the published manuscript and supporting online material. The information provided in the current ... -
Recombination Based Part Assembly
(2010-12-05)Here we propose a new recombination-based assembly standard, optimized to allow for efficient cloning of mammalian vectors.