Now showing items 1-3 of 3

    • BBF RFC 105: The Intein standard - a universal way to modify proteins after translation 

      Ahlmann-Eltze, Constantin; Bunne, Charlotte; Büscher, Magdalena; Gleixner, Jan; Horn, Max; e.a. (2015-03-17)
      This Request for Comments (RFC) proposes a new standard that allows for easy and flexible cloning of intein constructs and thus makes this technology accessible to the synthetic biology community.
    • BBF RFC 106: A Standard Type IIS Syntax for Plants 

      Rutten, Virginia; Munabi, Angelina; Riche, Fergus; Lewy, Guy; Wilson, Hugh; e.a. (2015-03-17)
      Here we define a standard syntax for assembling standard parts for expression in plant cells, extensible to all other eukaryotes. Variations of the Type IIS mediated cloning method known as Golden Gate Cloning, most notably ...
    • A Standard Parts List for Biological Circuitry 

      Arkin, Adam P; Endy, Drew (DARPA White Paper, 1999-10-07)
      One of the hallmarks of biochemical circuits found in nature is analog, asymmetric, asynchronous design. That is, there is little standardization of parts, e.g. all the promoters have different strengths and kinetics, ...