Now showing items 1-9 of 9
Design of Specific Mammalian Promoters by in silico Prediction
The purpose of this RFC is to provide a) a method for the design of rational synthetic promoter sequences based on a statistical analysis about the spatial preference of transcription factor binding sites in human promoter ...
HiCT: High Throughput Protocols For CPE Cloning And Transformation
The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...
BBF RFC 105: The Intein standard - a universal way to modify proteins after translation
This Request for Comments (RFC) proposes a new standard that allows for easy and flexible cloning of intein constructs and thus makes this technology accessible to the synthetic biology community.
RA-PCR, a method for the generation of randomized promoter libraries
The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of ...
Cloning Standard for Mammalian BioBrick Parts and Devices
To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.
miMeasure – a standard for miRNA binding site characterization in mammalian cells
This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...
Standard for Synthesis of Customized Peptides by Non-Ribosomal Peptide Synthetases
The purpose of this RFC is to introduce a standardized framework for the engineering of customizable non-ribosomal peptide synthetases (NRPS) and their application for in vivo and in vitro synthesis of short non-ribosomal ...
miTuner - a kit for microRNA based gene expression tuning in mammalian cells
The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ...
Units for Promoter Measurement in Mammalian Cells
The purpose of this RFC is to provide units for the characterization of promoter strength for use in mammalian cells. RMPU is mRNA based and directly proportional to PoPS, whereas REU is protein based and not proportional ...