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GenBrick – A Rapid Multi-Part Assembly Method for BioBricks

Gasiūnaitė, Lina; Lewicka, Aleksandra; Pashkuleva, Hristiana; Villanueva, Hugo; Thornton, Harry; e.a. (2013-10-04)

This BioBricks Foundation Request for Comments (BBF RFC) describes a novel approach, GenBrick, for the rapid assembly of multiple BioBrick RFC10-compatible parts in a single reaction, with completely flexible part order, ...

Abbreviated BioBrick Prefix and Suffix for More Efficient Primer Design

Thing, Teoh Shao; Yasumoto, Shuhei; Nakamura, Tadashi; Saka, Takahiro; Torigata, Kousuke; e.a. (2010-10-31)

This Request for Comments (RFC) modifies the assembly standard for biological parts proposed in BBF RFC 10 by removing the NotI restriction site from the BioBrick Prefix and Suffix.

"BioSandwich", a homology-based assembly method using a library of standard parts

Abbas, Yassen; Crossman, Allan; Fletcher, Eugene; French, Chris; Gibson, Clare; e.a. (2011-10-17)

This Request for Comments (RFC) describes a strategy for using homology-based assembly methods to assemble parts from a library in any order.

BBF RFC 104: BrickClip – rapid assembly of multiple RFC10 BioBricks

Trubitsyna, Maryia; Chan, Karen; Cai, Yizhi; Elfick, Alistair; French, Chris (2015-03-17)

This BioBrick Foundation Request for Comments (BBF RFC 104) describes a new approach to multiple part DNA assembly – BrickClip, which does not require use of any restriction enzymes, nor cloning of the parts to specific ...

Instructions to BBF RFC Authors

Tarjan, Daniel; Sleight, Sean; Shetty, Reshma; Ridgway, Doug; Rettberg, Randy; e.a. (OpenWetWare, 2008-11-10)

This BioBricks Foundation Request for Comments (BBF RFC) provides in- formation about the preparation and submission of BBF RFCs to The Bio- Bricks Foundation (BBF).