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RA-PCR, a method for the generation of randomized promoter libraries
(2009-10-21)
The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of ...
Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII
(2010-10-31)
With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at ...
Fast multiple gene fragment ligation method based on homologous recombination
(2010-10-31)
With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at ...