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Design of Specific Mammalian Promoters by in silico Prediction
The purpose of this RFC is to provide a) a method for the design of rational synthetic promoter sequences based on a statistical analysis about the spatial preference of transcription factor binding sites in human promoter ...
Cloning Standard for Mammalian BioBrick Parts and Devices
To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.
miMeasure – a standard for miRNA binding site characterization in mammalian cells
This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...
miTuner - a kit for microRNA based gene expression tuning in mammalian cells
The purpose of this RFC is to introduce a modular expression tuning kit for use in mammalian cells. The kit enables the regulation of the gene expression of any gene of interest (GOI) based on synthetic microRNAs, endogenous ...
Units for Promoter Measurement in Mammalian Cells
The purpose of this RFC is to provide units for the characterization of promoter strength for use in mammalian cells. RMPU is mRNA based and directly proportional to PoPS, whereas REU is protein based and not proportional ...