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Fluorescence Assay for Polymerase Arrival Rates
(2003-08-31)
To engineer complex synthetic biological systems will require modular design, assembly, and characterization strategies. The RNA polymerase arrival rate (PAR) is defined to be the rate that RNA polymerases arrive at a ...
Strategy for Biological Risk & Security
(2003-10)
Why do biological risks exist? Can we develop and implement a strategy for thoughtfully approaching future biological risks? This short, working report provides an abstract introduction to the problem of biological risk ...
Simulation, Models, and Refactoring of Bacteriophage T7
(2007-02-02)
Our understanding of why biological systems are designed in a particular way
would benefit from biophysically-realistic models that can make accurate predictions on the time-evolution of molecular events given arbitrary ...
Engineered Post-Translational Logic (PTL)
(2005-12-07)
Current synthetic biological circuits make use of protein-DNA and RNA-RNA interactions to control gene expression in bacteria. Systems that rely on the regulation of gene expression are relatively slow and unsuitable for ...
iGEM 2005 Invitation Supplement
(2005-09-29)
This document provides background material in support of the 2005 intercollegiate Genetically Engineered Machine Competition (aka iGEM). Herein, you can read quick summaries of earlier competitions and courses, obtain a ...
Refactoring Bacteriophage T7 (2004 version)
(2004-10)
Natural biological systems are selected by evolution to continue to exist. Evolution might
give rise to complicated systems that are difficult to discover, measure, model, and direct.
Here, we redesign the genome of a ...
BioJADE: A Design and Simulation Tool for Synthetic Biological Systems
(2004-05-28)
The next generations of both biological engineering and computer engineering demand that control be exerted at the molecular level. Creating, characterizing and controlling synthetic biological systems may provide us ...
Raw Data, Win MN, Smolke CD. 2008. Higher-order cellular information processing with synthetic RNA devices. Science. 322: 456-60. DOI: 10.1126/science.1160311
(2009-07-29)
This supplement provides additional detail on the data analysis methods and the raw data for the
RNA devices presented in the published manuscript and supporting online material. The
information provided in the current ...
DARPA BioComp Plasmid Distribution 1.00 of Standard Biobrick Components
(MIT Artificial Intelligence Laboratory, 2002-05-22)
This distribution consists of 300 ng aliquots of plasmid DNA for each of twelve components and compound constructs utilizing our idempotent assembly strategy. The dried DNA should be stable at room temperature for many ...
Idempotent Vector Design for Standard Assembly of Biobricks
(MIT Artificial Intelligence Laboratory; MIT Synthetic Biology Working Group, 2003)
The lack of standardization in assembly techniques for DNA sequences forces each DNA assembly
reaction to be both an experimental tool for addressing the current research topic, and an experiment
in and of itself. One ...