Now showing items 1-9 of 9
Assembly of BioBrick standard biological parts using three antibiotic assembly
(Elsevier Inc., 2011-05-20)
An underlying goal of synthetic biology is to make the process of engineering biological systems easier and more reliable. In support of this goal, we developed BioBrick assembly standard 10 to enable the construction of ...
BioBricks++: Simplifying Assembly of Standard DNA Components
Construction of complex biological systems can require assembling many modules together. However, existing assembly schemes are lacking in generality, ease of use, or power to perform some desirable operations. Currently, ...
Reusable rapid assembly of genetic parts for Neurospora crassa
This BBF RFC provides a method in which standardized parts can be easily created for Neurospora crassa.
HiCT: High Throughput Protocols For CPE Cloning And Transformation
The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...
GenBrick – A Rapid Multi-Part Assembly Method for BioBricks
This BioBricks Foundation Request for Comments (BBF RFC) describes a novel approach, GenBrick, for the rapid assembly of multiple BioBrick RFC10-compatible parts in a single reaction, with completely flexible part order, ...
PCR - Ligation Assembly Standard for BioBrick Parts
This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This ...
The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly
BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ...
Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units
BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note ...
"BioSandwich", a homology-based assembly method using a library of standard parts
This Request for Comments (RFC) describes a strategy for using homology-based assembly methods to assemble parts from a library in any order.