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Quantitative measurement of mammalian cell invasion by bacteria using flow cytometry
Purpose of this RFC is to provide method of measurement of bacterial invasion into mammalian cells. Also this RFC defines Percent of INvasion (PIN) unit.
The Measurement of rho‐independent Transcription Terminator Efficiency
The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of terminators in bacteria. Because we have characterized the performance of terminator in E. coli and used a simple ...
Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR
The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, ...
Units for Promoter Measurement in Mammalian Cells
The purpose of this RFC is to provide units for the characterization of promoter strength for use in mammalian cells. RMPU is mRNA based and directly proportional to PoPS, whereas REU is protein based and not proportional ...