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On-chip multi-dimensional biomolecule separation using multi-layer microfabricated valves

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dc.contributor.advisor Jongyoon Han. en_US
dc.contributor.author Wang, Ying-Chih, 1977- en_US
dc.contributor.other Massachusetts Institute of Technology. Dept. of Mechanical Engineering. en_US
dc.date.accessioned 2005-09-06T21:57:37Z
dc.date.available 2005-09-06T21:57:37Z
dc.date.copyright 2004 en_US
dc.date.issued 2004 en_US
dc.identifier.uri http://hdl.handle.net/1721.1/27143
dc.description Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004. en_US
dc.description Includes bibliographical references (p. 89-94). en_US
dc.description.abstract Recent proteomics researches require a sensitive, high-speed, and automatic protein separation tool that far exceeds the capability of traditional two dimensional (2D) protein gel electrophoresis. Methods are described to achieve multi-dimensional biomolecule separation in a microfluidic channel. The new methods couple isoelectric focusing (IEF) with high ionic strength capillary electrophoresis (CE) by active micro valve control in a microchip. Several experiments demonstrating independent 2D separation were performed, and critical parameters for better chip performance were identified; including channel passivation, electroosmosis control, IEF linearity control, and detection enhancement. The result can be used for the filtration of high-abundance proteins, which used to be done by affinity columns. Also, it can be used for much possible integration between different heterogeneous separation/analysis techniques such as IEF, polyacrylamide gel electrophoresis (PAGE), CE, reverse-phase chromatography, and mass spectrometry (MS). en_US
dc.description.statementofresponsibility by Ying-Chih Wang. en_US
dc.format.extent 94 p. en_US
dc.format.extent 3775156 bytes
dc.format.extent 3775156 bytes
dc.format.mimetype application/pdf
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. en_US
dc.rights.uri http://dspace.mit.edu/handle/1721.1/7582
dc.subject Mechanical Engineering. en_US
dc.title On-chip multi-dimensional biomolecule separation using multi-layer microfabricated valves en_US
dc.type Thesis en_US
dc.description.degree S.M. en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Mechanical Engineering. en_US
dc.identifier.oclc 56889960 en_US


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