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Active Hypothermic Growth: A Novel Means For Increasing Total Interferon-γ Production by Chinese Hamster Ovary Cells

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dc.contributor.author Stephen R., Fox
dc.contributor.author Yap, Mei Xia
dc.contributor.author Yap, Miranda G.S.
dc.contributor.author Wang, Daniel I.C.
dc.date.accessioned 2005-12-16T14:28:35Z
dc.date.available 2005-12-16T14:28:35Z
dc.date.issued 2006-01
dc.identifier.uri http://hdl.handle.net/1721.1/30379
dc.description.abstract When grown under hypothermic conditions, Chinese Hamster Ovary (CHO) cells become growth arrested in the G₀/G₁ phase of the cell cycle and also often exhibit increased recombinant protein production. In this study, we have validated this hypothesis by stimulating hypothermic growth using basic fibroblast growth factor and fetal bovine serum supplementation. This method led to 7.7- and 4.9-fold increase in total production compared to the 37°C and 32°C control cultures, respectively. This proof-of-concept study will motivate the creation of cell lines capable of growing at low temperatures for use in industrial processes. en
dc.description.sponsorship Singapore-MIT Alliance (SMA) en
dc.format.extent 192577 bytes
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Molecular Engineering of Biological and Chemical Systems (MEBCS) en
dc.subject Hypothermic growth en
dc.subject CHO cell en
dc.subject Interferon gamma en
dc.subject specific productivity en
dc.title Active Hypothermic Growth: A Novel Means For Increasing Total Interferon-γ Production by Chinese Hamster Ovary Cells en
dc.type Article en


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