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Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly

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dc.contributor.author Ajikumar, Parayil Kumaran
dc.contributor.author Ng, Jin Kiat
dc.contributor.author Tang, Yew Chung
dc.contributor.author Lee, Jim Yang
dc.contributor.author Stephanopoulos, Gregory
dc.contributor.author Too, Heng-Phon
dc.date.accessioned 2005-12-16T14:42:45Z
dc.date.available 2005-12-16T14:42:45Z
dc.date.issued 2006-01
dc.identifier.uri http://hdl.handle.net/1721.1/30384
dc.description.abstract In the post genomic era, proteomics has enormous potential in biology and medicine. Among the various bioanalytical tools developed, protein microarray is one of the recent advancements which offer high throughput profiling of cellular proteins to provide insights into the mechanisms of biological processes. Fundamentally, the protein microarray involves the immobilization of interacting elements, proteins, on a few square microns of a solid support and in principle, it is capable of detecting analytes with a higher sensitivity than conventional macroscopic immunoassays. Here in the present report we delineates the design, fabrication and functional analysis of protein microarray using semi-synthetic ssDNA tagged-proteins as capturing moiety as well as address on a solid support. Optimization of the platform has been carried out by investigating various parameters such as surface chemistry, signal amplification, and conditions for homogenous liguid phase protein-protein interaction. en
dc.description.sponsorship Singapore-MIT Alliance (SMA) en
dc.format.extent 131181 bytes
dc.format.mimetype application/pdf
dc.language.iso en en
dc.relation.ispartofseries Molecular Engineering of Biological and Chemical Systems (MEBCS) en
dc.subject Proteomics en
dc.subject Protein micro array en
dc.subject Dendrimer en
dc.subject glass slide en
dc.subject ssDNA-antibody en
dc.title Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly en
dc.type Article en


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