Inhibition of Hsp90 in Streptomyces coelicolor

Inhibition of Hsp90 in Streptomyces coelicolor

Alternative Title: Inhibition of Hsp90 in S. coelicolor

Author: Wu, Katherine A. (Katherine Ann)

Citable URI: http://hdl.handle.net/1721.1/36280

Other Contributors: Massachusetts Institute of Technology. Dept. of Chemistry.

Advisor: Sarah E. O'Connor.

Department: Massachusetts Institute of Technology. Dept. of Chemistry.

Publisher: Massachusetts Institute of Technology

Date Issued: 2005

Abstract:

Inhibition of the chaperone protein Hsp90 in plants and insects has been found to result in drastic changes in phenotype. We investigated the effect of Hsp90 inhibition on the bacteria Streptomyces coelicolor. These changes were studied by growing S. coelicolor in the presence of Hsp90 inhibitors, extracting the growth media, and subjecting the extractions to HPLC and MS analysis. It has been found that reproducibility is difficult to achieve. Since the genome of S. coelicolor has been sequenced, attention was turned to studying changes in mRNA expression levels, which gave more reproducible results, as well as insight to the specific genes being affected.

Description:

Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2005.Includes bibliographical references (leaves 20-21).

URI: http://hdl.handle.net/1721.1/36280

Keywords: Chemistry.

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