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Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture

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dc.contributor.author Yuk, Inn Huam Yvonne.
dc.contributor.author Wang, Daniel I.C.
dc.date.accessioned 2003-12-08T15:03:31Z
dc.date.available 2003-12-08T15:03:31Z
dc.date.issued 2003-01
dc.identifier.uri http://hdl.handle.net/1721.1/3776
dc.description.abstract Glycosylation of recombinant proteins can change during the culture of animal cells. Since lipid-linked oligosaccharides (LLOs) are the carbohydrate donors in N-linked glycosylation, their availability is postulated to influence the extent of glycosylation. To test this hypothesis, relative LLO and glycosylation levels in Chinese hamster ovary (CHO) cells were monitored over the course of batch culture for corresponding changes. Radiolabelling studies reveal that throughout the length of culture, intracellular LLO levels remained within a 2-fold range and overall CHO protein glycosylation varied by less than 30%. The implications of these results and hypotheses to explain the findings are discussed. en
dc.description.sponsorship Singapore-MIT Alliance (SMA) en
dc.format.extent 11281124 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.relation.ispartofseries Molecular Engineering of Biological and Chemical Systems (MEBCS);
dc.subject G0G1 phase en
dc.subject lipid-linked oligosaccharide en
dc.title Changes in the Overall Extent of Protein Glycosylation by Chinese Hamster Ovary Cells Over the Course of Batch Culture en
dc.type Article en


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