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A microfluidic platform for three-dimensional neuron culture

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dc.contributor.advisor Roger D. Kamm. en_US Varner, Johanna (Johanna M.) en_US
dc.contributor.other Massachusetts Institute of Technology. Biological Engineering Division. en_US 2008-01-10T16:01:05Z 2008-01-10T16:01:05Z 2007 en_US 2007 en_US
dc.description Thesis (M. Eng.)--Massachusetts Institute of Technology, Biological Engineering Division, 2007. en_US
dc.description Includes bibliographical references (p. 51-53). en_US
dc.description.abstract Neurodegenerative diseases typically affect a limited number of specific neuronal subtypes, and the death of these neurons causes permanent loss of a specific motor function. Efforts to restore function would require regenerating the affected cells, but progress is limited by a narrow understanding of the mechanisms that underlie the generation of these neurons from their progenitor cells. In order to prevent neuronal degeneration and potentially repair or regenerate the damaged motor output circuitry, it will be necessary to understand the molecular and genetic factors that control, direct, and enhance differentiation, axonal projection and connectivity. While techniques are available to separate specific populations of neurons once they are fully-differentiated, current methods make it nearly impossible to monitor or control the development of a neural precursor in standard open culture. To carry out directed differentiation experiments effectively, it will be critical to control how signals are introduced to the cells. In this study, we present a microfluidic system to address the limitations of previous research. en_US
dc.description.abstract (cont.) The device is capable of generating a controlled gradient of chemoattractant or growth factor of interest and directing axonal growth through an extra-cellular matrix material. Once the cells have grown into the device, signals and gradients can be applied directly to either the cell bodies or the axons. This device will serve as a platform technology for future experimentation with biomaterial scaffolds for neural tissue engineering, drug design or testing, and eventually directed differentiation of neural precursor cells. en_US
dc.description.statementofresponsibility by Johanna Varner. en_US
dc.format.extent 64 p. en_US
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. en_US
dc.subject Biological Engineering Division. en_US
dc.title A microfluidic platform for three-dimensional neuron culture en_US
dc.title.alternative microfluidic platform for 3-D neuron culture en_US
dc.type Thesis en_US M.Eng. en_US
dc.contributor.department Massachusetts Institute of Technology. Biological Engineering Division. en_US
dc.identifier.oclc 182621699 en_US

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