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Browsing Requests for Comments (RFCs) by Title

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  • Haddock, Traci L.; Densmore, Douglas M.; Appleton, Evan; Carr, Swati; Iverson, Sonya; De Freitas, Monique; Jin, Shawn; Awtry, Jake; Desai, Devina; Lozanoski, Thomas; Shah, Pooja; Agarwal, Yash; Lewis, Kathleen; Pacheco, Alan (2015-08-28)
    This RFC94 describes an assembly standard based on the Type IIS restriction enzymes BsaI and BbsI (also called BpiI). This assembly standard is based upon the Modular Cloning (MoClo) assembly strategy, which was introduced ...
  • Wang, Lingjue; Zhu, Xiaodan; Li, Hanrun; He, Huiyu; Ni, Zhuangdian; Zhou, Liqun (2013-10-29)
    Current, there is well adopted documentation of biobricks. The Registry of Standard Biology Part set the standard documentation for biobrick. However, there is no standard way to document a genetic circuit, what information ...
  • Abbas, Yassen; Crossman, Allan; Fletcher, Eugene; French, Chris; Gibson, Clare; Isapanie, Sylvia; Kopec, Lukasz; Lee, Mun Ching; Li, Di; Tynan-O'Mahony, Fionn (2011-10-17)
    This Request for Comments (RFC) describes a strategy for using homology-based assembly methods to assemble parts from a library in any order.
  • Mina, Petros; Savery, Nigel (2009-10-21)
    Protein fusions are currently not supported by most assembly standards. This assembly standard aims to provide the basis of a solution to a biobrick-friendly protein fusion mechanism that supports the current favorite ...
  • Balint, Balint L; Keret, Ophir; Brazda, Peter; Demeny, Mate; 2010 Debrecen-Hungary, iGEM (2010-12-05)
    The purpose of this RFC is to describe a method that allows the design of protein domain based parts, starting with gene centered information and translate these informations into BBF RFC 25 compatible part. The method is ...
  • Bartoschek, Michael; Mugahid, Douaa; Rademacher, Anne; Meyer, Hannah; Velten, Lars; Reis, Yara; Keienburg, Jens; Eils, Roland (2009-10-21)
    To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.
  • Che, Austin (2009-03-30)
    This Request for Comments (RFC) modifies the assembly standard for biological parts proposed in BBF RFC 10 by replacing PstI with SbfI and dropping other sequence constraints. This change relaxes the constraints on the ...
  • Lee, Hsiao-Ching (2010-12-05)
    The first step in programming and controlling cell behavior is to establish a library of well-defined components, a.k.a. "biobricks", that serve as the building blocks of artificial gene networks. The main challenge in ...
  • Grunberg, Raik (2009-03-30)
    This Request for Comments (RFC) describes a strategy for converting Biobricks from the Freiburg (aka Fusion) format to the Silver lab (aka BioFusion) format.
  • Xu, Kenneth O.; Walters, Eric M. (2011-12-29)
    The current registry system accommodates normal parts fairly well but has difficulty when adding mutant libraries. If all mutant offspring were added as new parts, the registry would be comprehensive but the parts registry ...
  • Heinemann, Tim; Kramer, Stephen; Velten, Lars; Kranz, Anna-Lena; Bauer, Tobias; Faura, Marti Bernardo; Konig, Rainer; Keienburg, Jens; Eils, Roland; Iwamoto, Nao (2009-10-30)
    The purpose of this RFC is to provide a) a method for the design of rational synthetic promoter sequences based on a statistical analysis about the spatial preference of transcription factor binding sites in human promoter ...
  • Culviner, Peter; Pantalone, Nathaniel; Sagstetter, Mary; Sandock, Sarah; Vrana, Justin; Wu, Yue (2010-12-05)
    The purpose of this standard is to increase the required information that must accompany any parts submitted to the Registry of Standard Biological Parts. This will give users of the parts better assurance of their ...
  • Slomp, Arend; Ekkers, David (2010-12-05)
    Purpose of this RFC is to improve the information supplied by BioBrick part vendors. Reason to have this RFC is that there would be much more information available which can help improve the search time of the user. ...
  • Knight, Tom (OpenWetWare, 2008-11-19)
    This standard defines the required sequence properties for a Biobrick(R) BB-2 standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards.
  • Knight, Tom (OpenWetWare, 2007-05-03)
    This standard defines the required sequence properties for a Biobrick(tm) standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards. ...
  • Fortuna, Patrick; Trolle, Thomas; Borch, Martin Malthe; Haugaard, Anastasiya; Kjaergaard, Maya Friis; Iversen, Lisa Blanc; Jollmann, Annemi; Sander, Anja; Frederiksen, Juliet; Slodkowicz, Grzegorz (2010-12-04)
    The purpose of this RFC is to outline a method for generating a BioBrick compatible Synthetic Promoter Library (SPL) within bacteria in order to fine-tune the expression of BioBrick parts and devices.
  • Wang, Ruiyan; Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
    With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at ...
  • Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
    With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at ...
  • Grünberg, Raik; Arndt, Katja; Müller, Kristian (2009-04-18)
    This Request for Comments (RFC) describes an extension to the original BioBrick assembly standard (BBF RFC 10). The Fusion Assembly strategy described here is fully backwards compatible with RFC 10 and supports the ...
  • Gasiūnaitė, Lina; Lewicka, Aleksandra; Pashkuleva, Hristiana; Villanueva, Hugo; Thornton, Harry; Trubitsyna, Maryia; French, Chris (2013-10-04)
    This BioBricks Foundation Request for Comments (BBF RFC) describes a novel approach, GenBrick, for the rapid assembly of multiple BioBrick RFC10-compatible parts in a single reaction, with completely flexible part order, ...
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