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Browsing Requests for Comments (RFCs) by Title

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  • Bartoschek, Michael; Mugahid, Douaa; Rademacher, Anne; Meyer, Hannah; Velten, Lars; Reis, Yara; Keienburg, Jens; Eils, Roland (2009-10-21)
    To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.
  • Che, Austin (2009-03-30)
    This Request for Comments (RFC) modifies the assembly standard for biological parts proposed in BBF RFC 10 by replacing PstI with SbfI and dropping other sequence constraints. This change relaxes the constraints on the ...
  • Lee, Hsiao-Ching (2010-12-05)
    The first step in programming and controlling cell behavior is to establish a library of well-defined components, a.k.a. "biobricks", that serve as the building blocks of artificial gene networks. The main challenge in ...
  • Grunberg, Raik (2009-03-30)
    This Request for Comments (RFC) describes a strategy for converting Biobricks from the Freiburg (aka Fusion) format to the Silver lab (aka BioFusion) format.
  • Xu, Kenneth O.; Walters, Eric M. (2011-12-29)
    The current registry system accommodates normal parts fairly well but has difficulty when adding mutant libraries. If all mutant offspring were added as new parts, the registry would be comprehensive but the parts registry ...
  • Heinemann, Tim; Kramer, Stephen; Velten, Lars; Kranz, Anna-Lena; Bauer, Tobias; Faura, Marti Bernardo; Konig, Rainer; Keienburg, Jens; Eils, Roland; Iwamoto, Nao (2009-10-30)
    The purpose of this RFC is to provide a) a method for the design of rational synthetic promoter sequences based on a statistical analysis about the spatial preference of transcription factor binding sites in human promoter ...
  • Culviner, Peter; Pantalone, Nathaniel; Sagstetter, Mary; Sandock, Sarah; Vrana, Justin; Wu, Yue (2010-12-05)
    The purpose of this standard is to increase the required information that must accompany any parts submitted to the Registry of Standard Biological Parts. This will give users of the parts better assurance of their ...
  • Slomp, Arend; Ekkers, David (2010-12-05)
    Purpose of this RFC is to improve the information supplied by BioBrick part vendors. Reason to have this RFC is that there would be much more information available which can help improve the search time of the user. ...
  • Knight, Tom (OpenWetWare, 2008-11-19)
    This standard defines the required sequence properties for a Biobrick(R) BB-2 standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards.
  • Knight, Tom (OpenWetWare, 2007-05-03)
    This standard defines the required sequence properties for a Biobrick(tm) standard biological part. It does not define any functional characteristics of the parts, nor does it motivate any aspect of these standards. ...
  • Fortuna, Patrick; Trolle, Thomas; Borch, Martin Malthe; Haugaard, Anastasiya; Kjaergaard, Maya Friis; Iversen, Lisa Blanc; Jollmann, Annemi; Sander, Anja; Frederiksen, Juliet; Slodkowicz, Grzegorz (2010-12-04)
    The purpose of this RFC is to outline a method for generating a BioBrick compatible Synthetic Promoter Library (SPL) within bacteria in order to fine-tune the expression of BioBrick parts and devices.
  • Wang, Ruiyan; Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
    With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at ...
  • Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
    With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at ...
  • Grünberg, Raik; Arndt, Katja; Müller, Kristian (2009-04-18)
    This Request for Comments (RFC) describes an extension to the original BioBrick assembly standard (BBF RFC 10). The Fusion Assembly strategy described here is fully backwards compatible with RFC 10 and supports the ...
  • Gasiūnaitė, Lina; Lewicka, Aleksandra; Pashkuleva, Hristiana; Villanueva, Hugo; Thornton, Harry; Trubitsyna, Maryia; French, Chris (2013-10-04)
    This BioBricks Foundation Request for Comments (BBF RFC) describes a novel approach, GenBrick, for the rapid assembly of multiple BioBrick RFC10-compatible parts in a single reaction, with completely flexible part order, ...
  • Billerbeck, Sonja; Zwicky, Katharina; Lang, Moritz; Pestalozzi, Luzius; Rosenberger, George; Constantinescu, Simona; Ambegoda, Thanuja; Sotiriadis, Elsa (2010-12-05)
    BBF RFC 75 intends to link the BBF RFC 28-based combinatorial multi-part assembly method to BBF RFC 10. Its aim is to easily redesign an expression vector to make it compatible to BBF RFC 10 for the construction of fusion ...
  • Pauthenier, Cyrille; El-Sayyed, Hafez; Rostain, William; Cerisy, Tristan; Lopez, Carolina Gallo; Ferreira, Raphael; Moreira, Jorgelindo Da Veiga; Souterre, Tiffany; Eeckhout, Joachim; Nogue, Pierre Yves; Llamosi, Artemis; Parutto, Pierre; Nikolayeva, Iryna; Machat, Mohamed; Marquet, Clement; Młynarczyk, Anna; Thelie, Aurore; Landrain, Thomas; Tolonen, Andrew; Pollet, Nicolas; Faulon, Jean-Loup; Jaramilo, Alfonso (2012-11-05)
    BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ...
  • Beer, Ralf; Christiansen, Tania; Herbst, Konrad; Ignatiadis, Nikolaos; Kats, Ilia; Kurzawa, Nils; Meichsner, Johanna; Rabe, Sophie; Riedel, Anja; Sachs, Joshua; Schessner, Julia; Schmidt, Florian; Walch, Philipp; Adlung, Lorenz; Genereith, Katharina; Georgi, Fanny; Heinemann, Tim; Meyer, Hannah; Niopek, Dominik; Di Ventura, Barbara; Eils, Roland (2013-10-04)
    The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...
  • Weeding, Emma; Houle, Jason; Swiniarski, Ben; Smadbeck, Patrick; Lindblad, Kristen; Volzing, Katherine; Srivastava, Poonam; Sotiropoulos, Vassilios; Biliouris, Kostas; Kaznessis, Yiannis (2009-10-21)
    This BioBricks Foundation Request for Comments (BBF RFC) provides instructions on how to automatically generate a model of any BioBrick sequence. These models can be used in computer simulations of the dynamic behavior of ...
  • Tarjan, Daniel; Sleight, Sean; Shetty, Reshma; Ridgway, Doug; Rettberg, Randy; McArthur, George; Mayer, Antonia; Knight, Tom; French, Chris; Endy, Drew; de Mora, Kim; Elfick, Alistair; Cowell, Mackenzie; Che, Austin; Anderson, Chris (OpenWetWare, 2008-11-10)
    This BioBricks Foundation Request for Comments (BBF RFC) provides in- formation about the preparation and submission of BBF RFCs to The Bio- Bricks Foundation (BBF).
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