Now showing items 21-40 of 74

    • Bioscaffold-Linker 

      Mina, Petros; Savery, Nigel (2009-10-21)
      Protein fusions are currently not supported by most assembly standards. This assembly standard aims to provide the basis of a solution to a biobrick-friendly protein fusion mechanism that supports the current favorite ...
    • BBF RFC 47: BioBytes Assembly Standard 

      Ellison, Michael; Ridgway, Doug; Fedor, Justin; Garside, Erin; Robinson, Kelly; e.a. (2009-10-29)
      The BioBytes assembly method provides a new high-throughput way of assembling plasmids as well as other large DNA constructs. Using a bead-linked assembly of genetic parts with long (12 bp) standardized sticky ends, ...
    • Design of Specific Mammalian Promoters by in silico Prediction 

      Heinemann, Tim; Kramer, Stephen; Velten, Lars; Kranz, Anna-Lena; Bauer, Tobias; e.a. (2009-10-30)
      The purpose of this RFC is to provide a) a method for the design of rational synthetic promoter sequences based on a statistical analysis about the spatial preference of transcription factor binding sites in human promoter ...
    • Large-Scale Peptide Modification on BioBrick Proteins 

      Tian, Feng; Chen, GuoQiang; Wang, Zhao (2009-10-30)
      With the established BioBrick Assembly standards, fusion proteins with diversified functions are easy to manipulate. However, we realized that the existing BBF RFC do not clearly defining the standard for peptide display ...
    • The USER cloning standard 

      Kaas, Christian Schrøder; Hansen, Niels Bjørn; Genee, Hans Jasper; Olsen, Lars Rønn; Matos, Cláudia; e.a. (2009-11-01)
      This BioBricks Foundation Request for Comments (BBF RFC) provides information about the design of uracil-containing primers used for USER cloning and USER fusion.
    • BBF RFC 16: Synthetic Biology Open Language Visual (SBOLv) Specification 

      Rodriguez, Cesar; Bartram, Suzie; Ramasubramanian, Anusuya; Endy, Drew (2009-11-01)
      Synthetic Biology Open Language Visual (SBOLv) is a graphical notation that supports biological device development. It provides a formal notation for describing the physical composition of basic parts into composite ...
    • BBF RFC 56: “Part Flavors” For Peptide-Coding Parts 

      Anderson, J. Chris (2010-10-11)
      “Part Flavors” are a means of describing the grammatical properties of parts. Specifically, this is introduced as a means of describing parts that encode peptides (CDS parts), but there may be value to extrapolating the ...
    • Quantitative measurement of mammalian cell invasion by bacteria using flow cytometry 

      Lower, Michal; Olchowik, Anna (2010-10-31)
      Purpose of this RFC is to provide method of measurement of bacterial invasion into mammalian cells. Also this RFC defines Percent of INvasion (PIN) unit.
    • Abbreviated BioBrick Prefix and Suffix for More Efficient Primer Design 

      Thing, Teoh Shao; Yasumoto, Shuhei; Nakamura, Tadashi; Saka, Takahiro; Torigata, Kousuke; e.a. (2010-10-31)
      This Request for Comments (RFC) modifies the assembly standard for biological parts proposed in BBF RFC 10 by removing the NotI restriction site from the BioBrick Prefix and Suffix.
    • Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII 

      Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
      With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 61 is developed aimed at ...
    • Fast multiple gene fragment ligation method based on homologous recombination 

      Wang, Ruiyan; Shi, Zhenyu; Li, Teng; Chen, Guoqiang (2010-10-31)
      With the established BioBrick Assembly standards, ligation of different parts has to be accomplished step by step. It can be time-consuming when dealing with multiple fragment ligation. BBF RFC 62 is developed aimed at ...
    • Promoter and Coding Sequence Considerations for Caenorhabditis elegans and Other Eukaryotes 

      Palmer, Chris; Halliday, Geoff (2010-11-07)
      Our primary aim is to provide a standard format for protein-coding BioBricks intended for eukaryotes, especially in the nematode worm Caenorhabditis elegans, so that future teams will be able to correctly take advantage ...
    • Standard Biological Part Automatic Modeling Database Language (MoDeL) 

      Wang, Zhen; Liao, Chen; Jiang, Hao; Yao, Xiaomo; Jiang, Kun (2010-11-09)
      This BioBricks Foundation Request for Comments (BBF RFC) describes the Standard Biological Part Automatic Modeling Database Language (MoDeL). MoDeL provides a language and syntax standard for automatic modeling databases ...
    • DTU Synthetic Promoter Library Standard 

      Fortuna, Patrick; Trolle, Thomas; Borch, Martin Malthe; Haugaard, Anastasiya; Kjaergaard, Maya Friis; e.a. (2010-12-04)
      The purpose of this RFC is to outline a method for generating a BioBrick compatible Synthetic Promoter Library (SPL) within bacteria in order to fine-tune the expression of BioBrick parts and devices.
    • Open licensing of BioBrick parts 

      Lower, Michal; Olchowik, Anna; Pankowski, Jaroslaw; Bazlekowa, Milena; Blaszkiewicz, Marta; e.a. (2010-12-05)
      This document provides recommendations for licensing of community-created biological parts, especially in BioBrick standard.
    • Standard for the Electronic Distribution of SBOLv Diagrams 

      Johnson, Jeffrey; Galdzicki, Michal; Sauro, Herbert M (2010-12-05)
      A common method for publicly distributing design diagrams on the Web would enhance understanding of the mechanisms and intended function of synthetic DNA constructs. We aim to improve the usefulness of the depicted design ...
    • Documentation of BioBrick parts 

      Slomp, Arend; Ekkers, David (2010-12-05)
      Purpose of this RFC is to improve the information supplied by BioBrick part vendors. Reason to have this RFC is that there would be much more information available which can help improve the search time of the user. ...
    • Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR 

      Olchowik, Anna; Lower, Michal; Moreno, Cherry; Pankowski, Jaroslaw (2010-12-05)
      The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, ...
    • General updating scheme for the design and construction of assembly vectors that are compatible with BBF RFC 10 and BBF RFC 28 

      Billerbeck, Sonja; Zwicky, Katharina; Lang, Moritz; Pestalozzi, Luzius; Rosenberger, George; e.a. (2010-12-05)
      BBF RFC 75 intends to link the BBF RFC 28-based combinatorial multi-part assembly method to BBF RFC 10. Its aim is to easily redesign an expression vector to make it compatible to BBF RFC 10 for the construction of fusion ...
    • miMeasure – a standard for miRNA binding site characterization in mammalian cells 

      Adlung, Lorenz; Sabah, Jude Al; Bayer, Philipp; Berrens, Rebecca; Cristiano, Elena; e.a. (2010-12-05)
      This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, ...