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Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering

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dc.contributor.advisor Myron Spector. en_US
dc.contributor.author Ueda, Erica (Erica Ann) en_US
dc.contributor.other Massachusetts Institute of Technology. Dept. of Mechanical Engineering. en_US
dc.date.accessioned 2009-03-16T19:51:20Z
dc.date.available 2009-03-16T19:51:20Z
dc.date.copyright 2008 en_US
dc.date.issued 2008 en_US
dc.identifier.uri http://hdl.handle.net/1721.1/44853
dc.description Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2008. en_US
dc.description Includes bibliographical references (p. 123-125). en_US
dc.description.abstract Rat neural stem cells (NSCs) were cultured in monolayer or in porous collagen scaffolds and exposed to neurogenic or non-neurogenic medium to determine the effects on neural differentiation and neurite growth. Nestin, [beta]III-tubulin, and GFAP expression were determined using immunofluorescent techniques, and the neurite length was measured. NSCs differentiated into neurons, with actively growing neurites, and astrocytes when cultured in differentiation medium (DM) or neurogenic medium (NM). NSCs cultured in monolayer expressed more nestin and III-tubulin and had significantly longer neurite extensions than NSCs cultured in collagen scaffolds. Laminin coated scaffolds promoted the attachment of NSCs to the scaffold struts and resulted in a more even distribution of nestin and [beta]III-tubulin positive cells throughout the scaffold. Overall, NSCs cultured in DM for at least 14 days resulted in the most neuronal differentiation and neurite growth. en_US
dc.description.statementofresponsibility by Erica Ueda. en_US
dc.format.extent 125 p. en_US
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. en_US
dc.rights.uri http://dspace.mit.edu/handle/1721.1/7582 en_US
dc.subject Mechanical Engineering. en_US
dc.title Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering en_US
dc.type Thesis en_US
dc.description.degree S.M. en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Mechanical Engineering. en_US
dc.identifier.oclc 301735192 en_US


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