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Nanofluidic system for single molecule manipulation and analysis

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dc.contributor.advisor Rohit N. Karnik. en_US
dc.contributor.author Sen, Yi-Heng en_US
dc.contributor.other Massachusetts Institute of Technology. Dept. of Mechanical Engineering. en_US
dc.date.accessioned 2009-08-26T16:48:13Z
dc.date.available 2009-08-26T16:48:13Z
dc.date.copyright 2008 en_US
dc.date.issued 2008 en_US
dc.identifier.uri http://hdl.handle.net/1721.1/46547
dc.description Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2008. en_US
dc.description Includes bibliographical references (p. 55-58). en_US
dc.description.abstract This thesis focuses on characterizing and controlling the translocation of single 48.5 kbp [lambda]-DNA molecules through an artificial nanopore with the objective of enabling multiple measurements on the same molecule. This approach may enable nanopore sensors with enhanced size or charge resolution through statistical averaging over multiple detection events. Nanopores with dimensions of 200 nm x 500 nm x 5 pm connected by microfluidic channels were fabricated using soft lithography in polydimethylsiloxane (PDMS). The PDMS nanopore could successfully detect translocation events of single [lambda]DNA molecules. Factors such as applied voltage bias, DNA concentration, and dimensions of the channel were found to affect the frequency of translocation events and signal-to-noise ratio, which are critical factors for implementing multiple measurements on the same molecule with feedback control. Noise contributions from each part of the experimental apparatus and device were also characterized. Feedback control using Labview was implemented to reverse the direction of applied voltage bias upon detection of a translocation event. The direction of travel of single DNA molecules could be successfully reversed and two measurements on the same molecule were realized. This work lays the foundations for a nanofluidic device for enhanced measurement resolution through statistical averaging over multiple measurements on the same molecule. en_US
dc.description.statementofresponsibility by Yi-Heng Sen. en_US
dc.format.extent 58 p. en_US
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. en_US
dc.rights.uri http://dspace.mit.edu/handle/1721.1/7582 en_US
dc.subject Mechanical Engineering. en_US
dc.title Nanofluidic system for single molecule manipulation and analysis en_US
dc.type Thesis en_US
dc.description.degree S.M. en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Mechanical Engineering. en_US
dc.identifier.oclc 418251683 en_US


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