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BBF RFC 21: BglBricks Assembly Standard

Research and Teaching Output of the MIT Community

Show simple item record Anderson, J Christopher Dueber, John E Leguia, Mariana Wu, Gabriel C Goler, Jonathan A Arkin, Adam P Keasling, Jay D 2009-09-18T14:38:40Z 2009-09-18T14:38:40Z 2009-09-18T14:38:40Z
dc.description.abstract The BglBricks standard has been developed as an alternative to the original XbaI/SpeI standard primarily to provide a solution to the issue of generating translational-fusion parts. When joined together in a standard assembly reaction, BglBricks parts contain a scar sequence of GGATCT between the two parts. This sequence conveniently translates as Gly-Ser in the zero frame, a commonly-used linker in protein engineering. BglBricks has previously been called the “BglBrick” standard, and is also referred to as Assembly standard 21. A consolidated description of the standard is available at: en
dc.language.iso en_US en
dc.relation.ispartofseries BBF RFC;21
dc.subject assembly standard en
dc.title BBF RFC 21: BglBricks Assembly Standard en
dc.type Technical Report en

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