BBF RFC 21: BglBricks Assembly Standard

dc.contributor.author	Anderson, J Christopher
dc.contributor.author	Dueber, John E
dc.contributor.author	Leguia, Mariana
dc.contributor.author	Wu, Gabriel C
dc.contributor.author	Goler, Jonathan A
dc.contributor.author	Arkin, Adam P
dc.contributor.author	Keasling, Jay D
dc.date.accessioned	2009-09-18T14:38:40Z
dc.date.available	2009-09-18T14:38:40Z
dc.date.issued	2009-09-18T14:38:40Z
dc.identifier.uri	http://hdl.handle.net/1721.1/46747
dc.description.abstract	The BglBricks standard has been developed as an alternative to the original XbaI/SpeI standard primarily to provide a solution to the issue of generating translational-fusion parts. When joined together in a standard assembly reaction, BglBricks parts contain a scar sequence of GGATCT between the two parts. This sequence conveniently translates as Gly-Ser in the zero frame, a commonly-used linker in protein engineering. BglBricks has previously been called the “BglBrick” standard, and is also referred to as Assembly standard 21. A consolidated description of the standard is available at: http://openwetware.org/wiki/Template:AndersonLab:BglBrick_Standard	en
dc.language.iso	en_US	en
dc.relation.ispartofseries	BBF RFC;21
dc.subject	assembly standard	en
dc.title	BBF RFC 21: BglBricks Assembly Standard	en
dc.type	Technical Report	en