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BBF RFC 21: BglBricks Assembly Standard

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dc.contributor.author Anderson, J Christopher
dc.contributor.author Dueber, John E
dc.contributor.author Leguia, Mariana
dc.contributor.author Wu, Gabriel C
dc.contributor.author Goler, Jonathan A
dc.contributor.author Arkin, Adam P
dc.contributor.author Keasling, Jay D
dc.date.accessioned 2009-09-18T14:38:40Z
dc.date.available 2009-09-18T14:38:40Z
dc.date.issued 2009-09-18T14:38:40Z
dc.identifier.uri http://hdl.handle.net/1721.1/46747
dc.description.abstract The BglBricks standard has been developed as an alternative to the original XbaI/SpeI standard primarily to provide a solution to the issue of generating translational-fusion parts. When joined together in a standard assembly reaction, BglBricks parts contain a scar sequence of GGATCT between the two parts. This sequence conveniently translates as Gly-Ser in the zero frame, a commonly-used linker in protein engineering. BglBricks has previously been called the “BglBrick” standard, and is also referred to as Assembly standard 21. A consolidated description of the standard is available at: http://openwetware.org/wiki/Template:AndersonLab:BglBrick_Standard en
dc.language.iso en_US en
dc.relation.ispartofseries BBF RFC;21
dc.subject assembly standard en
dc.title BBF RFC 21: BglBricks Assembly Standard en
dc.type Technical Report en


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