BBF RFC 21: BglBricks Assembly Standard
Name
BBFRFC21.pdf
Size
19.66 KB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Anderson, J Christopher
Dueber, John E
Leguia, Mariana
Wu, Gabriel C
Goler, Jonathan A
Arkin, Adam P
Keasling, Jay D
Date Issued
September 18, 2009
Series/Report no.
BBF RFC;21
Abstract
The BglBricks standard has been developed as an alternative to the original XbaI/SpeI standard primarily to provide a solution to the issue of generating translational-fusion parts. When joined together in a standard assembly reaction, BglBricks parts contain a scar sequence of GGATCT between the two parts. This sequence conveniently translates as Gly-Ser in the zero frame, a commonly-used linker in protein engineering. BglBricks has previously been called the “BglBrick” standard, and is also referred to as Assembly standard 21. A consolidated description of the standard is available at: http://openwetware.org/wiki/Template:AndersonLab:BglBrick_Standard
Subjects
assembly standard
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