Browsing MIT Open Access Articles by Author "Yacoby, Iftach"
Now showing items 1-2 of 2
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Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase
Yacoby, Iftach; Tegler, Lotta Tollstoy; Pochekailov, Sergii; Zhang, Shuguang; King, Paul W. (Public Library of Science, 2012-04)Background: Recombinant expression and purification of metallo-enzymes, including hydrogenases, at high-yields is challenging due to complex, and enzyme specific, post-translational maturation processes. Low fidelities ... -
Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin:NADP+-oxidoreductase (FNR) enzymes in vitro
Yacoby, Iftach; Pochekailov, Sergii; Zhang, Shuguang; Toporik, Hila; Ghirardi, Maria L.; e.a. (Proceedings of the National Academy of Sciences (PNAS), 2011-05)Photosynthetic water splitting, coupled to hydrogenase-catalyzed hydrogen production, is considered a promising clean, renewable source of energy. It is widely accepted that the oxygen sensitivity of hydrogen production, ...