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RA-PCR, a method for the generation of randomized promoter libraries

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dc.contributor.author Velten, Lars
dc.contributor.author Haas, Simon
dc.contributor.author Rademacher, Anne
dc.contributor.author Meyer, Hannah
dc.contributor.author Eils, Roland
dc.date.accessioned 2009-10-21T01:24:36Z
dc.date.available 2009-10-21T01:24:36Z
dc.date.issued 2009-10-21T01:24:36Z
dc.identifier.uri http://hdl.handle.net/1721.1/49502
dc.description.abstract The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of transcription factors of interest). The method generates a library of promoters putatively active under the desired condition. This RFC also provides instructions on how to screen the libraries generated by this method in order to obtain functional promoters. Description of promoters generated by this method can be found at http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters. en
dc.language.iso en en
dc.relation.ispartofseries BBF RFC;42
dc.subject method en
dc.title RA-PCR, a method for the generation of randomized promoter libraries en
dc.type Technical Report en


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