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The 5' inositol phosphatase SHIP2 regulates EGF-elicited protrusion in MTLn3 cells

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dc.contributor.advisor Frank B. Gertler. en_US Kuhlmann, Georgiana L. (Georgiana Louise) en_US
dc.contributor.other Massachusetts Institute of Technology. Dept. of Biology. en_US 2010-09-02T17:25:30Z 2010-09-02T17:25:30Z 2010 en_US 2010 en_US
dc.description Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Biology, 2010. en_US
dc.description Cataloged from PDF version of thesis. en_US
dc.description Includes bibliographical references (p. 47-57). en_US
dc.description.abstract In metastatic cancer, cells must be able to migrate from their original environment, move through the blood or lymphatic system, and colonize a distant organ. Mena, a member of the Ena/VASP family of proteins, is upregulated in invasive populations of breast cancer cells. The Ena/VASP (enabled/vasodilator-stimulated phosphoprotein) family of proteins regulate both the geometry and dynamics of actin filament networks. Mena specifically is alternatively spliced with an invasive isoform, MenaINV, upregulated in metastastic cells, while an epithelial isoform, Menalla, is downregulated. SH2- domain containing 5-inositol phosphatase (SHIP2) interacts with Mena and is thought to play a role in breast cancer. SHIP2 is a 5-phosphatase that catalyzes the dephosphorylation of phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P 3) to phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2) as well as the dephosphorylation of phosphatidylinositol 4,5- bisphosphate (PI(4,5)P 2). PI(3,4,5)P 3 and P][(4,5)P 2 are two major phosphoinositides at the plasma membrane and regulate a variety of cellular functions, including receptor signaling, membrane-cytoskeleton interactions and clathrin-mediated endocytosis. Here I have looked at the effects of knocking down SHIP2 in the MTLn3 cell line, a metastatic rat breast carcinoma line. I found that when SHIP2 is knocked down in cells, there is an increase in membrane protrusion upon stimulation with EGF, and that recruitment of Mena to the leading edge is enhanced, implying that this increase in protrusion may be due to a change in Mena localization. en_US
dc.description.statementofresponsibility by Georgiana L. Kuhlmann. en_US
dc.format.extent 57 p. en_US
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. en_US
dc.rights.uri en_US
dc.subject Biology. en_US
dc.title The 5' inositol phosphatase SHIP2 regulates EGF-elicited protrusion in MTLn3 cells en_US
dc.title.alternative Effects of SHIP2 knockdown in MTLn3 cells en_US
dc.type Thesis en_US S.M. en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Biology. en_US
dc.identifier.oclc 654432791 en_US

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