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Label-free classification of cell types by imaging of cell membrane fluctuations using low-coherent full-field quantitative phase microscopy

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Label-free classification of cell types by imaging of cell membrane fluctuations using low-coherent full-field quantitative phase microscopy

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Author: Yamauchi, Toyohiko; Sugiyama, Norikazu; Sakurai, Takashi; Iwai, Hidenao; Yamashita, Yutaka
Department: Massachusetts Institute of Technology. Spectroscopy Laboratory
Publisher: SPIE
Date Issued: 2010-02
Abstract:
Cell membrane motions of living cells are quantitatively measured in nanometer resolution by low-coherent full-field quantitative phase microscopy. Our setup is based on a full-field phase shifting interference microscope with a very lowcoherent light source. The reflection mode configuration and the low-coherent illumination make it possible to differentiate the weak reflection light from the cell membrane from the strong reflection from the glass substrate. Two cell populations are quantitatively assessed by the power spectral density of the cell surface motion and show different trends.
URI: http://hdl.handle.net/1721.1/58576
ISSN: 0277-786X
Citation: Yamauchi, Toyohiko et al. “Label-free classification of cell types by imaging of cell membrane fluctuations using low-coherent full-field quantitative phase microscopy.” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII. Ed. Jose-Angel Conchello et al. San Francisco, California, USA: SPIE, 2010. 75700X-8. ©2010 SPIE.
Version: Final published version
Terms of Use: Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Published As: http://dx.doi.org/10.1117/12.841008
Journal: Proceedings of SPIE--the International Society for Optical Engineering ; v. 7570

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