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miMeasure – a standard for miRNA binding site characterization in mammalian cells

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dc.contributor.author Adlung, Lorenz
dc.contributor.author Sabah, Jude Al
dc.contributor.author Bayer, Philipp
dc.contributor.author Berrens, Rebecca
dc.contributor.author Cristiano, Elena
dc.contributor.author Flocke, Lea
dc.contributor.author Kleinsorg, Stefan
dc.contributor.author Kolodziejczyk, Aleksandra
dc.contributor.author Kraemer, Stephen
dc.contributor.author Torre, Alejandro Macias
dc.contributor.author Mathur, Aastha
dc.contributor.author Mayilo, Dmytro
dc.contributor.author Neumann, Stefan
dc.contributor.author Niopek, Dominik
dc.contributor.author Pisa, Rudolf
dc.contributor.author Schad, Jan-Ulrich
dc.contributor.author Schumacher, Laura-Nadine
dc.contributor.author Uhlig, Thomas
dc.contributor.author Wu, Xiaoting
dc.contributor.author Keienburg, Jens
dc.contributor.author Boerner, Kathleen
dc.contributor.author Grimm, Dirk
dc.contributor.author Eils, Roland
dc.date.accessioned 2010-12-05T21:10:08Z
dc.date.available 2010-12-05T21:10:08Z
dc.date.issued 2010-12-05
dc.identifier.uri http://hdl.handle.net/1721.1/60092
dc.description.abstract This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, BBa_K337049) enabling rapid experimental characterization of any miRNA-BS of choice. We recommend a new standard unit, RKDU (relative knock-down unit) to describe the knock-down efficiency of a miRNA-BS in a specific cell type. pSMB_miMeasure allows for an easy and fast measurement of RKDU while providing effective normalization against variance stemming from differences in transfection efficiency and from other sources. en_US
dc.language.iso en en_US
dc.relation.ispartofseries BBF RFC;73
dc.rights Attribution-No Derivative Works 3.0 United States en
dc.rights.uri http://creativecommons.org/licenses/by-nd/3.0/us/ en
dc.subject measurement standard en_US
dc.title miMeasure – a standard for miRNA binding site characterization in mammalian cells en_US
dc.type Technical Report en_US


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