Structural Basis of Regiospecificity of a Mononuclear Iron Enzyme in Antibiotic Fosfomycin Biosynthesis
Author(s)
Yun, Danny; Dey, Mishtu; Higgins, Luke J.; Yan, Feng; Liu, Hung-wen; Drennan, Catherine L; ... Show more Show less
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Hydroxypropylphosphonic acid epoxidase (HppE) is an unusual mononuclear iron enzyme that uses dioxygen to catalyze the oxidative epoxidation of (S)-2-hydroxypropylphosphonic acid (S-HPP) in the biosynthesis of the antibiotic fosfomycin. Additionally, the enzyme converts the R-enantiomer of the substrate (R-HPP) to 2-oxo-propylphosphonic acid. To probe the mechanism of HppE regiospecificity, we determined three X-ray structures: R-HPP with inert cobalt-containing enzyme (Co(II)–HppE) at 2.1 Å [angstrom] resolution; R-HPP with active iron-containing enzyme (Fe(II)–HppE) at 3.0 Å [angstrom] resolution; and S-HPP–Fe(II)–HppE in complex with dioxygen mimic NO at 2.9 Å [angstrom] resolution. These structures, along with previously determined structures of S-HPP–HppE, identify the dioxygen binding site on iron and elegantly illustrate how HppE is able to recognize both substrate enantiomers to catalyze two completely distinct reactions.
Date issued
2011-06Department
Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of ChemistryJournal
Journal of the American Chemical Society
Publisher
American Chemical Society
Citation
Yun, Danny et al. “Structural Basis of Regiospecificity of a Mononuclear Iron Enzyme in Antibiotic Fosfomycin Biosynthesis.” Journal of the American Chemical Society 133.29 (2011) : 11262-11269. Copyright © 2011 American Chemical Society
Version: Final published version
ISSN
0002-7863
1520-5126