Towards a carbon nanotube antibody sensor
Name
795184338-MIT.pdf
Description
Full printable version
Size
10.95 MB
Format
Adobe PDF
Checksum (MD5)
787b2ad055b659a1356c51164184074c
Author(s)
Bojö, Peter
Advisor(s)
Michael Strano.
Date Issued
2012
Publisher
Massachusetts Institute of Technology
Abstract
This work investigated single-walled carbon nanotube (SWNT)/polymer-protein A complexes for optically reporting antibody concentration via a change in near infrared fluorescent emission after antibody binding. SWNT have potential as biosensors because of extraordinary sensitivity, lack of photobleaching, and optical activity in a near-infrared window. A SWNT sensor could provide label-free measurements of antibody concentration in a continuous fashion, which may aid selection of production strains. Protein A itself, dextran, poly vinyl alcohol, DNA sequences, and chitosan were used as polymers for wrapping SWNT. Nonspecific binding to solution-phase constructs was found to be a major problem with these approaches. Chitosan hydrogels encapsulating SWNT also show nonspecific responses.
Description
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, February 2012.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 46-51).
Subjects
Biological Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.
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