Advanced Search

Physiological effects of heterologous expression of proteorhodopsin photosystems

Research and Teaching Output of the MIT Community

Show simple item record

dc.contributor.advisor Edward F. De long. en_US Buck, Justin David en_US
dc.contributor.other Massachusetts Institute of Technology. Dept. of Biological Engineering. en_US 2012-07-02T15:43:19Z 2012-07-02T15:43:19Z 2012 en_US 2012 en_US
dc.description Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, 2012. en_US
dc.description Cataloged from PDF version of thesis. en_US
dc.description Includes bibliographical references (p. 175-195). en_US
dc.description.abstract Proteorhodopsin (PR) phototrophy plays an important role in the marine ecosystem, harvesting energy from sunlight for a diverse community of hetertrophic organisms. The simple proteorhodopsin photosystem (PRPS) composed of six to seven genes is sufficient for producing a functional light-driven proton pump, capable of powering cellular processes. This thesis describes the functional characterization of a subcloned PRPS previously identified from a large insert metagenomic library (Martinez et al., 2007). Incorporation of the PRPS into a strain of Pseudomonas putida resulted in a light-dependent increase in viable cell yield of cultures grown in low carbon media. The light-dependent effect demonstrates a dependence on carbon, reducing at increasing carbon concentrations until no differential effect is observed. A survey of six PR-containing vectors from metagenomic libraries revealed PR transcription in two hosts, P. putida and Pseudoalteromonas atlantica, and of the three additional vectors with PRPS tested, two demonstrated the same qualitative light-dependent yield increase. This work illustrates the utility of a simple rhodopsin photosystem for supplementing the cellular energy system of a heterologous host, paving the way for future engineering applications in photoheterotrophy. en_US
dc.description.statementofresponsibility by Justin David Buck. en_US
dc.format.extent 195 p. en_US
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. en_US
dc.rights.uri en_US
dc.subject Biological Engineering. en_US
dc.title Physiological effects of heterologous expression of proteorhodopsin photosystems en_US
dc.type Thesis en_US Ph.D. en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Biological Engineering. en_US
dc.identifier.oclc 795184381 en_US

Files in this item

Name Size Format Description
795184381-MIT.pdf 15.65Mb PDF Full printable version

This item appears in the following Collection(s)

Show simple item record