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Macromolecular Crowding Directs Extracellular Matrix Organization and Mesenchymal Stem Cell Behavior

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dc.contributor.author Zeiger, Adam Scott
dc.contributor.author Loe, Felicia C.
dc.contributor.author Li, Ran
dc.contributor.author Raghunath, Michael
dc.contributor.author Van Vliet, Krystyn J. Van
dc.date.accessioned 2012-07-17T12:51:01Z
dc.date.available 2012-07-17T12:51:01Z
dc.date.issued 2012-05
dc.date.submitted 2012-04
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/1721.1/71642
dc.description.abstract Microenvironments of biological cells are dominated in vivo by macromolecular crowding and resultant excluded volume effects. This feature is absent in dilute in vitro cell culture. Here, we induced macromolecular crowding in vitro by using synthetic macromolecular globules of nm-scale radius at physiological levels of fractional volume occupancy. We quantified the impact of induced crowding on the extracellular and intracellular protein organization of human mesenchymal stem cells (MSCs) via immunocytochemistry, atomic force microscopy (AFM), and AFM-enabled nanoindentation. Macromolecular crowding in extracellular culture media directly induced supramolecular assembly and alignment of extracellular matrix proteins deposited by cells, which in turn increased alignment of the intracellular actin cytoskeleton. The resulting cell-matrix reciprocity further affected adhesion, proliferation, and migration behavior of MSCs. Macromolecular crowding can thus aid the design of more physiologically relevant in vitro studies and devices for MSCs and other cells, by increasing the fidelity between materials synthesized by cells in vivo and in vitro. en_US
dc.description.sponsorship Massachusetts Institute of Technology. Singapore-MIT Alliance in Research and Technology (SMART) en_US
dc.description.sponsorship National Defense Science and Engineering Graduate Fellowship en_US
dc.description.sponsorship National Institutes of Health (U.S.). Molecular, Cell, and Tissue Biomechanics Training Grant (5T32EB006348-05) en_US
dc.description.sponsorship National Science Foundation (U.S.). Materials Research Science and Engineering Centers (Program) (DMR-0819762) en_US
dc.description.sponsorship National University of Singapore. Faculty Research Committee Grant en_US
dc.description.sponsorship National University of Singapore. Tissue Engineering Programme en_US
dc.description.sponsorship National Science Foundation (U.S.). (CAREER Award CBET-0644846) en_US
dc.language.iso en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof http://dx.doi.org/10.1371/journal.pone.0037904 en_US
dc.rights Creative Commons Attribution en_US
dc.source PLoS en_US
dc.title Macromolecular Crowding Directs Extracellular Matrix Organization and Mesenchymal Stem Cell Behavior en_US
dc.type Article en_US
dc.identifier.citation Zeiger, Adam S. et al. “Macromolecular Crowding Directs Extracellular Matrix Organization and Mesenchymal Stem Cell Behavior.” Ed. Effie C. Tsilibary. PLoS ONE 7.5 (2012): e37904. en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Materials Science and Engineering en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Biological Engineering en_US
dc.contributor.approver Van Vliet, Krystyn J.
dc.contributor.mitauthor Zeiger, Adam Scott
dc.contributor.mitauthor Loe, Felicia C.
dc.contributor.mitauthor Li, Ran
dc.contributor.mitauthor Raghunath, Michael
dc.contributor.mitauthor Van Vliet, Krystyn J.
dc.relation.journal PLoS ONE en_US
dc.identifier.mitlicense PUBLISHER_POLICY en_US
dc.eprint.version Final published version en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dspace.orderedauthors Zeiger, Adam S.; Loe, Felicia C.; Li, Ran; Raghunath, Michael; Van Vliet, Krystyn J. en


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