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2D protrusion but not motility predicts growth factor–induced cancer cell migration in 3D collagen

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dc.contributor.author Meyer, Aaron Samuel
dc.contributor.author Hughes-Alford, Shannon Kay
dc.contributor.author Kay, Jennifer Elizabeth
dc.contributor.author Castillo, Amalchi
dc.contributor.author Wells, Alan
dc.contributor.author Gertler, Frank
dc.contributor.author Lauffenburger, Douglas A.
dc.date.accessioned 2012-07-27T14:45:36Z
dc.date.available 2012-07-27T14:45:36Z
dc.date.issued 2012-06
dc.date.submitted 2012-01
dc.identifier.issn 0021-9525
dc.identifier.issn 1540-8140
dc.identifier.uri http://hdl.handle.net/1721.1/71869
dc.description.abstract Growth factor–induced migration is a critical step in the dissemination and metastasis of solid tumors. Although differences in properties characterizing cell migration on two-dimensional (2D) substrata versus within three-dimensional (3D) matrices have been noted for particular growth factor stimuli, the 2D approach remains in more common use as an efficient surrogate, especially for high-throughput experiments. We therefore were motivated to investigate which migration properties measured in various 2D assays might be reflective of 3D migratory behavioral responses. We used human triple-negative breast cancer lines stimulated by a panel of receptor tyrosine kinase ligands relevant to mammary carcinoma progression. Whereas 2D migration properties did not correlate well with 3D behavior across multiple growth factors, we found that increased membrane protrusion elicited by growth factor stimulation did relate robustly to enhanced 3D migration properties of the MDA-MB-231 and MDA-MB-157 lines. Interestingly, we observed this to be a more reliable relationship than cognate receptor expression or activation levels across these and two additional mammary tumor lines. en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Grant no. R01- GM081336) en_US
dc.description.sponsorship National Science Foundation (U.S.). Graduate Research Fellowship en_US
dc.description.sponsorship United States. Dept. of Defense. Congressionally Directed Medical Research Programs. Breast Cancer Research Program (Grant no. W81XWH-11-1-0088) en_US
dc.description.sponsorship United States. Dept. of Defense. Congressionally Directed Medical Research Programs. Breast Cancer Research Program (Grant no. W81XWH-10-1-0040) en_US
dc.language.iso en_US
dc.publisher Rockefeller University Press, The en_US
dc.relation.isversionof http://dx.doi.org/10.1083/jcb.201201003 en_US
dc.rights Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ en_US
dc.source Rockefeller UP en_US
dc.title 2D protrusion but not motility predicts growth factor–induced cancer cell migration in 3D collagen en_US
dc.type Article en_US
dc.identifier.citation Meyer, A. S. et al. “2D Protrusion but Not Motility Predicts Growth Factor-induced Cancer Cell Migration in 3D Collagen.” The Journal of Cell Biology 197.6 (2012): 721–729. Copyright © 2012 by The Rockefeller University Press en_US
dc.contributor.department David H. Koch Institute for Integrative Cancer Research at MIT en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Biological Engineering en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Biology en_US
dc.contributor.approver Lauffenburger, Douglas A.
dc.contributor.mitauthor Meyer, Aaron Samuel
dc.contributor.mitauthor Hughes-Alford, Shannon Kay
dc.contributor.mitauthor Kay, Jennifer Elizabeth
dc.contributor.mitauthor Castillo, Amalchi
dc.contributor.mitauthor Gertler, Frank
dc.contributor.mitauthor Lauffenburger, Douglas A.
dc.relation.journal Journal of Cell Biology en_US
dc.identifier.mitlicense PUBLISHER_CC en_US
dc.eprint.version Final published version en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dspace.orderedauthors Meyer, A. S.; Hughes-Alford, S. K.; Kay, J. E.; Castillo, A.; Wells, A.; Gertler, F. B.; Lauffenburger, D. A. en


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