Advanced Search
DSpace@MIT

Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units

Research and Teaching Output of the MIT Community

Show simple item record

dc.contributor.author Boeke, Jef
dc.contributor.author Mitchell, Leslie
dc.contributor.author Cai, Yizhi
dc.contributor.author Stracquadanio, Giovanni
dc.contributor.author Chuang, James
dc.contributor.author Tan, Scott
dc.contributor.author Heston, Margo
dc.contributor.author Wang, Jiarui
dc.contributor.author Kim, Dong Won
dc.contributor.author Noronha, Anne Marie
dc.date.accessioned 2012-10-12T02:40:06Z
dc.date.available 2012-10-12T02:40:06Z
dc.date.issued 2012-10-11
dc.identifier.uri http://hdl.handle.net/1721.1/73912
dc.description.abstract BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note that we use the term “promoter” here to refer to both the promoter and the 5’ UTR, which we currently define as a single part. Future iterations of this standard will incorporate subdivision of currently defined parts e.g. into promoter and 5’ UTR. The standard makes use of the type IIS restriction enzyme BsaI to generate standardized and user­‐defined ‘signature overhangs’, thus enabling directional and seamless TU assembly. RFC88 is supported by the Yeast Standardized Collection of Parts for Expression (SCoPE), a repository of subcloned and sequence verified parts compatible with this assembly standard. The Yeast SCoPE is currently populated by a large number of S. cerevisiae promoters and terminators that facilitate expression and characterization of non­‐native ORFs. en_US
dc.language.iso en_US en_US
dc.relation.ispartofseries BBF RFC;88
dc.rights Attribution-NoDerivs 3.0 United States en
dc.rights.uri http://creativecommons.org/licenses/by-nd/3.0/us/ en
dc.subject DNA assembly en_US
dc.subject yeast en_US
dc.title Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units en_US
dc.type Technical Report en_US


Files in this item

Name Size Format Description
BBFRFC88.pdf 538.4Kb PDF

The following license files are associated with this item:

This item appears in the following Collection(s)

Show simple item record

Attribution-NoDerivs 3.0 United States Except where otherwise noted, this item's license is described as Attribution-NoDerivs 3.0 United States
MIT-Mirage