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Efficient shRNA-Mediated Inhibition of Gene Expression in Zebrafish

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dc.contributor.author Sive, Hazel L.
dc.contributor.author Rienzo, Gianluca De
dc.contributor.author Gutzman, Jennifer H.
dc.date.accessioned 2012-11-13T16:21:16Z
dc.date.available 2012-11-13T16:21:16Z
dc.date.issued 2012-07
dc.identifier.issn 1545-8547
dc.identifier.issn 1557-8542
dc.identifier.uri http://hdl.handle.net/1721.1/74628
dc.description.abstract Despite the broad repertoire of loss of function (LOF) tools available for use in the zebrafish, there remains a need for a simple and rapid method that can inhibit expression of genes at later stages. RNAi would fulfill that role, and a previous report (Dong et al. 2009) provided encouraging data. The goal of this study was to further address the ability of expressed shRNAs to inhibit gene expression. This included quantifying RNA knockdown, testing specificity of shRNA effects, and determining whether tissue-specific LOF could be achieved. Using an F0 transgenic approach, this report demonstrates that for two genes, wnt5b and zDisc1, each with described mutant and morphant phenotypes, shRNAs efficiently decrease endogenous RNA levels. Phenotypes elicited by shRNA resemble those of mutants and morphants, and are reversed by expression of cognate RNA, further demonstrating specificity. Tissue-specific expression of zDisc1 shRNAs in F0 transgenics demonstrates that conditional LOF can be readily obtained. These results suggest that shRNA expression presents a viable approach for rapid inhibition of zebrafish gene expression. en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Grant R01 DE021109) en_US
dc.language.iso en_US
dc.publisher Mary Ann Liebert en_US
dc.relation.isversionof http://dx.doi.org/10.1089/zeb.2012.0770 en_US
dc.rights Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. en_US
dc.source Mary Ann Liebert en_US
dc.title Efficient shRNA-Mediated Inhibition of Gene Expression in Zebrafish en_US
dc.type Article en_US
dc.identifier.citation De Rienzo, Gianluca, Jennifer H. Gutzman, and Hazel Sive. “Efficient shRNA-Mediated Inhibition of Gene Expression in Zebrafish.” Zebrafish 9.3 (2012): 97–107. ©2012 Mary Ann Liebert, Inc. publishers. en_US
dc.contributor.department Massachusetts Institute of Technology. School of Science en_US
dc.contributor.department Whitehead Institute for Biomedical Research en_US
dc.contributor.mitauthor Sive, Hazel L.
dc.relation.journal Zebrafish en_US
dc.identifier.mitlicense PUBLISHER_POLICY en_US
dc.eprint.version Final published version en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dspace.orderedauthors De Rienzo, Gianluca; Gutzman, Jennifer H.; Sive, Hazel en


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