dc.contributor.author | Maly, Thorsten | |
dc.contributor.author | Andreas, Loren | |
dc.contributor.author | Griffin, Robert Guy | |
dc.contributor.author | Smith, Albert A. | |
dc.date.accessioned | 2012-11-13T16:50:29Z | |
dc.date.available | 2012-11-13T16:50:29Z | |
dc.date.issued | 2010-05 | |
dc.date.submitted | 2010-03 | |
dc.identifier.issn | 1463-9076 | |
dc.identifier.issn | 1463-9084 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/74630 | |
dc.description.abstract | Perdeuteration of biological macromolecules for magic angle spinning solid-state NMR spectroscopy can yield high-resolution [superscript 2]H–[superscript 13]C correlation spectra and the method is therefore of great interest for the structural biology community. Here we demonstrate that the combination of sample deuteration and dynamic nuclear polarization yields resolved [superscript 2]H–[superscript 13]C correlation spectra with a signal enhancement of ε ≥ 700 compared to a spectrum recorded with microwaves off and otherwise identical conditions. To our knowledge, this is the first time that [superscript 2]2H-DNP has been employed to enhance MAS-NMR spectra of a biologically relevant system. The DNP process is studied using several polarizing agents and the technique is applied to obtain [superscript 2]H–[superscript 13]C correlation spectra of U-[[superscript 2]H, [superscript 13]C] proline. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant EB002084) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant EB002026) | en_US |
dc.description.sponsorship | German Research Foundation. Research Fellowship | en_US |
dc.description.sponsorship | National Science Foundation (U.S.). Graduate Research Fellowship Program | en_US |
dc.language.iso | en_US | |
dc.publisher | Royal Society of Chemistry, The | en_US |
dc.relation.isversionof | http://dx.doi.org/ 10.1039/c003705b | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike 3.0 | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
dc.source | Prof. Griffin via Erja Kajosalo | en_US |
dc.title | [superscript 2]H-DNP-enhanced [superscript 2]H-[superscript 13]C solid-state NMR correlation spectroscopy | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Maly, Thorsten et al. “2H-DNP-enhanced 2H–13C Solid-state NMR Correlation Spectroscopy.” Physical Chemistry Chemical Physics 12.22 (2010): 5872. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
dc.contributor.department | Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology) | en_US |
dc.contributor.approver | Griffin, Robert G. | |
dc.contributor.mitauthor | Maly, Thorsten | |
dc.contributor.mitauthor | Andreas, Loren | |
dc.contributor.mitauthor | Smith, Albert Andrew | |
dc.contributor.mitauthor | Griffin, Robert Guy | |
dc.relation.journal | Physical Chemistry Chemical Physics | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Maly, Thorsten; Andreas, Loren B.; Smith, Albert A.; Griffin, Robert G. | en |
dc.identifier.orcid | https://orcid.org/0000-0003-1589-832X | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |