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[superscript 2]H-DNP-enhanced [superscript 2]H-[superscript 13]C solid-state NMR correlation spectroscopy

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dc.contributor.author Maly, Thorsten
dc.contributor.author Andreas, Loren
dc.contributor.author Smith, Albert Andrew
dc.contributor.author Griffin, Robert Guy
dc.date.accessioned 2012-11-13T16:50:29Z
dc.date.available 2012-11-13T16:50:29Z
dc.date.issued 2010-05
dc.date.submitted 2010-03
dc.identifier.issn 1463-9076
dc.identifier.issn 1463-9084
dc.identifier.uri http://hdl.handle.net/1721.1/74630
dc.description.abstract Perdeuteration of biological macromolecules for magic angle spinning solid-state NMR spectroscopy can yield high-resolution [superscript 2]H–[superscript 13]C correlation spectra and the method is therefore of great interest for the structural biology community. Here we demonstrate that the combination of sample deuteration and dynamic nuclear polarization yields resolved [superscript 2]H–[superscript 13]C correlation spectra with a signal enhancement of ε ≥ 700 compared to a spectrum recorded with microwaves off and otherwise identical conditions. To our knowledge, this is the first time that [superscript 2]2H-DNP has been employed to enhance MAS-NMR spectra of a biologically relevant system. The DNP process is studied using several polarizing agents and the technique is applied to obtain [superscript 2]H–[superscript 13]C correlation spectra of U-[[superscript 2]H, [superscript 13]C] proline. en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Grant EB002084) en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Grant EB002026) en_US
dc.description.sponsorship German Research Foundation. Research Fellowship en_US
dc.description.sponsorship National Science Foundation (U.S.). Graduate Research Fellowship Program en_US
dc.language.iso en_US
dc.publisher Royal Society of Chemistry, The en_US
dc.relation.isversionof http://dx.doi.org/ 10.1039/c003705b en_US
dc.rights Creative Commons Attribution-Noncommercial-Share Alike 3.0 en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ en_US
dc.source Prof. Griffin via Erja Kajosalo en_US
dc.title [superscript 2]H-DNP-enhanced [superscript 2]H-[superscript 13]C solid-state NMR correlation spectroscopy en_US
dc.type Article en_US
dc.identifier.citation Maly, Thorsten et al. “2H-DNP-enhanced 2H–13C Solid-state NMR Correlation Spectroscopy.” Physical Chemistry Chemical Physics 12.22 (2010): 5872. en_US
dc.contributor.department Francis Bitter National Magnet Laboratory en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Chemistry en_US
dc.contributor.approver Griffin, Robert G.
dc.contributor.mitauthor Maly, Thorsten
dc.contributor.mitauthor Andreas, Loren
dc.contributor.mitauthor Smith, Albert Andrew
dc.contributor.mitauthor Griffin, Robert Guy
dc.relation.journal Physical Chemistry Chemical Physics en_US
dc.identifier.mitlicense OPEN_ACCESS_POLICY en_US
dc.eprint.version Author's final manuscript en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dspace.orderedauthors Maly, Thorsten; Andreas, Loren B.; Smith, Albert A.; Griffin, Robert G. en


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