Browsing Department of Biology by Title
Now showing items 673-692 of 1048
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The N-end rule degradation pathway : substrate recognition and staged delivery to the CIpAP protease by the CIpS adaptor protein
(Massachusetts Institute of Technology, 2011)Regulated protein degradation is crucial in the regulation of many physiological processes as well as in protein quality control. In all organisms, ranging from bacteria to mammals, ATP-dependent proteases carry out regulated ... -
A nanobody suite for yeast scaffold nucleoporins provides details of the Y complex structure and nuclear pore complex assembly
(Massachusetts Institute of Technology, 2020)Nuclear pore complexes (NPCs) are the main conduits for molecular exchange across the nuclear envelope. The NPC is a modular assembly of ~500 individual proteins, called nucleoporins or nups, that can be classified into ... -
Naturally occuring peptides recognized by cytotoxic T lymphocytes (CTS) : peptide abundance as a determinant of CTL function
(Massachusetts Institute of Technology, 1996) -
The necessity of debranching in starch biosynthesis
(Massachusetts Institute of Technology, 1999)Although research in starch biosynthesis has been carried out since the 1940s, many issues remain unresolved. For example, the number of reactions in the pathway is still an open question. The biosynthesis of the starch ... -
Negative feedback regulation as a means to constrain the oncogenic potential of mutant Egfr in NSCLC
(Massachusetts Institute of Technology, 2011)The discovery of EGFR kinase domain mutations in NSCLC patients who responded to tyrosine kinase inhibitors (TKIs) represented the first example of a targeted therapy for lung cancer. The dependence of these human tumors ... -
Neoblast specialization during regeneration of the planarian S. mediterranea
(Massachusetts Institute of Technology, 2018)Planarians are well known for their ability to regenerate an entire animal from small tissue fragments. Planarian regeneration requires a population of dividing cells called neoblasts that are distributed throughout the ... -
Neural correlates of locomotion, cues, and context in the interactions between hippocampus and lateral septum
(Massachusetts Institute of Technology, 2019)The lateral septum (LS) has been implicated in anxiety and fear modulation, and may regulate interactions between the hippocampus (HPC) and regions that mediate goal directed behavior. In this study, we simultaneously ... -
Neuronal growth cone responses to extracellular guidance cues : an in vitro study
(Massachusetts Institute of Technology, 1998) -
New insights on the ecology of free-living heterotrophic nanoflagellates based on the use of molecular biological approaches
(Massachusetts Institute of Technology, 1997) -
New mechanisms in transcription regulation and chromosome organization in Caulobacter crescentus
(Massachusetts Institute of Technology, 2016)Bacteria use a variety of mechanisms to control transcription in response to environmental cues or growth conditions. Activation or repression of transcription is often carried out by proteins, called transcription factors, ... -
New methods to study human mammary development and breast cancer
(Massachusetts Institute of Technology, 2017)Breast cancer is fundamentally a disease of aberrant differentiation. Breast cancers arise from within the normal architecture of the mammary gland and resemble normal mammary epithelial cell types on a molecular and gene ... -
New technologies and methodologies for the study of human mammary epithelial differentiation and development
(Massachusetts Institute of Technology, 2018)The mammary gland is unique among human tissues in that the bulk of its development occurs postnatally, under hormonal control. The gland consists of an epithelium that expands dramatically during puberty and pregnancy, ... -
Niche adaptations of the vibrionaceae, from the coastal ocean to the laboratory
(Massachusetts Institute of Technology, 2015)Microorganisms play a significant role in biogeochemical cycling, thus their dynamics in the environment influence the biosphere. Yet how do features of the environment - such as abiotic conditions, resources, and predators ... -
Normal microtubule function and the interaction between the pathways for tubulin folding and expression in S. cerevisiae
(Massachusetts Institute of Technology, 2003)Undimerized -tubulin is toxic to the yeast Saccharomyces cereivisiae. Free P-tubulin can arise if the tubulin heterodimer dissociates or if levels of 3-tubulin and cc-tubulin are unbalanced. I am using the toxicity of ... -
A novel approach for the study of sequences that control cytoplasmic RNA stability
(Massachusetts Institute of Technology, 1987) -
Novel methods in computational analysis and design of protein-protein interactions : applications to phosphoregulated interactions
(Massachusetts Institute of Technology, 2007)This thesis presents a number of novel computational methods for the analysis and design of protein-protein complexes, and their application to the study of the interactions of phosphopeptides with phosphopeptide-binding ... -
Novel regulatory mechanisms of mutagenic translesion DNA synthesis : characterization of REV1 in Saccharomyces cerevisiae
(Massachusetts Institute of Technology, 2007)Cells are constantly subjected to DNA damage from endogenous and exogenous sources. Spontaneous DNA damage alone accounts for -30,000 DNA lesions per day in a mammalian cell. Cells are also exposed to an enormous variety ... -
A novel role for the transcriptional modulator NusA in DNA repair/damage tolerance pathways in Escherichia coli
(Massachusetts Institute of Technology, 2009)All organisms must contend with the consequences of DNA damage, induced by a variety of both endogenous and exogenous sources. Mechanisms of DNA repair and DNA damage tolerance are crucial for cellular survival after DNA ... -
Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine
(Massachusetts Institute of Technology, 2015)ATP-powered proteases enforce protein quality-control and regulation in all domains of life. ClpX, a AAA+ ring homohexamer, uses the energy of ATP binding and hydrolysis to power conformational changes that unfold and ... -
Nucleotide recognition and metal ion requirements of an RNA ligase ribozyme
(Massachusetts Institute of Technology, 2003)The class I ligase, a ribozyme previously isolated from random sequence, catalyzes a reaction similar to RNA polymerization, positioning its 5'-nucleotide via a Watson-Crick base pair, forming a 3',5'-phosphodiester bond ...
