The importance and pervasiveness of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects ...

There is an ever-growing demand for enantiopure chemical compounds, particularly new pharmaceuticals. Enzymes, as natural biocatalysts, possess many appealing properties as robust asymmetric catalysts for synthetic chemistry. ...

Monoclonal antibodies have emerged as an important class of cancer therapeutics due to their ability to specifically bind tumor-expressed antigens. Unfortunately, attempts to treat solid tumors with these drugs are often ...

Optical traps have played a central role in the exploration of biological systems through the examination of molecular motors, biopolymers, and many other interactions at the nano and micro length scales. This thesis seeks ...

Biotherapeutics have revolutionized medicine with their ability to achieve unprecedented molecular recognition and mediate complex biological responses. The intracellular delivery of biotherapeutics is an unmet scientific ...

The potential of gene therapy to treat disease and improve human health is tremendous. The failure of viral gene therapy clinical trials due to toxicity, immunogenicity, and carcinogenicity has been tragic and strongly ...

The combination of optical tweezers and single molecule fluorescence into an instrument capable of making combined, coincident measurements adds an observable dimension that allows for the examination of the localized ...

To shed light on the cell's response to its mechanical environment, we examined cell rheology at the single cell level and quantified it with nanometer spatial and microsecond temporal resolutions over a five-decade frequency ...