Show simple item record

dc.contributor.authorScimone, M. Lucila
dc.contributor.authorLapan, Sylvain W.
dc.contributor.authorKravarik, Kellie Marie
dc.contributor.authorReddien, Peter
dc.contributor.authorScimone, M. Lucila
dc.contributor.authorLapan, Sylvain W.
dc.date.accessioned2015-04-02T16:58:56Z
dc.date.available2015-04-02T16:58:56Z
dc.date.issued2014-07
dc.date.submitted2014-06
dc.identifier.issn22136711
dc.identifier.urihttp://hdl.handle.net/1721.1/96336
dc.description.abstractPlanarians can regenerate any missing body part in a process requiring dividing cells called neoblasts. Historically, neoblasts have largely been considered a homogeneous stem cell population. Most studies, however, analyzed neoblasts at the population rather than the single-cell level, leaving the degree of heterogeneity in this population unresolved. We combined RNA sequencing of neoblasts from wounded planarians with expression screening and identified 33 transcription factors transcribed in specific differentiated cells and in small fractions of neoblasts during regeneration. Many neoblast subsets expressing distinct tissue-associated transcription factors were present, suggesting candidate specification into many lineages. Consistent with this possibility, klf, pax3/7, and FoxA were required for the differentiation of cintillo-expressing sensory neurons, dopamine-β-hydroxylase-expressing neurons, and the pharynx, respectively. Together, these results suggest that specification of cell fate for most-to-all regenerative lineages occurs within neoblasts, with regenerative cells of blastemas being generated from a highly heterogeneous collection of lineage-specified neoblasts.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (R01GM080639)en_US
dc.description.sponsorshipNational Science Foundation (U.S.). Graduate Research Fellowship Program (Grant 1122374)en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.stemcr.2014.06.001en_US
dc.rightsCreative Commons Attribution-Noncommercial-No deriviativesen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en_US
dc.sourceElsevieren_US
dc.titleNeoblast Specialization in Regeneration of the Planarian Schmidtea mediterraneaen_US
dc.typeArticleen_US
dc.identifier.citationScimone, M. Lucila, Kellie M. Kravarik, Sylvain W. Lapan, and Peter W. Reddien. “Neoblast Specialization in Regeneration of the Planarian Schmidtea Mediterranea.” Stem Cell Reports 3, no. 2 (August 2014): 339–352.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorKravarik, Kellie Marieen_US
dc.contributor.mitauthorReddien, Peteren_US
dc.contributor.mitauthorScimone, M. Lucilaen_US
dc.contributor.mitauthorLapan, Sylvain W.en_US
dc.relation.journalStem Cell Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsScimone, M. Lucila; Kravarik, Kellie M.; Lapan, Sylvain W.; Reddien, Peter W.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-5569-333X
dc.identifier.orcidhttps://orcid.org/0000-0001-9884-4663
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record