dc.contributor.author | Scimone, M. Lucila | |
dc.contributor.author | Lapan, Sylvain W. | |
dc.contributor.author | Kravarik, Kellie Marie | |
dc.contributor.author | Reddien, Peter | |
dc.contributor.author | Scimone, M. Lucila | |
dc.contributor.author | Lapan, Sylvain W. | |
dc.date.accessioned | 2015-04-02T16:58:56Z | |
dc.date.available | 2015-04-02T16:58:56Z | |
dc.date.issued | 2014-07 | |
dc.date.submitted | 2014-06 | |
dc.identifier.issn | 22136711 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/96336 | |
dc.description.abstract | Planarians can regenerate any missing body part in a process requiring dividing cells called neoblasts. Historically, neoblasts have largely been considered a homogeneous stem cell population. Most studies, however, analyzed neoblasts at the population rather than the single-cell level, leaving the degree of heterogeneity in this population unresolved. We combined RNA sequencing of neoblasts from wounded planarians with expression screening and identified 33 transcription factors transcribed in specific differentiated cells and in small fractions of neoblasts during regeneration. Many neoblast subsets expressing distinct tissue-associated transcription factors were present, suggesting candidate specification into many lineages. Consistent with this possibility, klf, pax3/7, and FoxA were required for the differentiation of cintillo-expressing sensory neurons, dopamine-β-hydroxylase-expressing neurons, and the pharynx, respectively. Together, these results suggest that specification of cell fate for most-to-all regenerative lineages occurs within neoblasts, with regenerative cells of blastemas being generated from a highly heterogeneous collection of lineage-specified neoblasts. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (R01GM080639) | en_US |
dc.description.sponsorship | National Science Foundation (U.S.). Graduate Research Fellowship Program (Grant 1122374) | en_US |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1016/j.stemcr.2014.06.001 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-No deriviatives | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | en_US |
dc.source | Elsevier | en_US |
dc.title | Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Scimone, M. Lucila, Kellie M. Kravarik, Sylvain W. Lapan, and Peter W. Reddien. “Neoblast Specialization in Regeneration of the Planarian Schmidtea Mediterranea.” Stem Cell Reports 3, no. 2 (August 2014): 339–352. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.mitauthor | Kravarik, Kellie Marie | en_US |
dc.contributor.mitauthor | Reddien, Peter | en_US |
dc.contributor.mitauthor | Scimone, M. Lucila | en_US |
dc.contributor.mitauthor | Lapan, Sylvain W. | en_US |
dc.relation.journal | Stem Cell Reports | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Scimone, M. Lucila; Kravarik, Kellie M.; Lapan, Sylvain W.; Reddien, Peter W. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-5569-333X | |
dc.identifier.orcid | https://orcid.org/0000-0001-9884-4663 | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |