Optimized E.coli expression strain LOBSTR eliminates common contaminants from His-tag purification
Author(s)
Andersen, Kasper R.; Leksa, Nina Carolina; Schwartz, Thomas
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His-tag affinity purification is one of the most commonly used methods to purify recombinant proteins expressed in E. coli. One drawback of using the His-tag is the co-purification of contaminating histidine-rich E. coli proteins. We engineered a new E. coli expression strain, LOBSTR (low background strain), which eliminates the most abundant contaminants. LOBSTR is derived from the E. coli BL21(DE3) strain and carries genomically modified copies of arnA and slyD, whose protein products exhibit reduced affinities to Ni and Co resins, resulting in a much higher purity of the target protein. The use of LOBSTR enables the pursuit of challenging low-expressing protein targets by reducing background contamination with no additional purification steps, materials, or costs, and thus pushes the limits of standard His-tag purifications.
Date issued
2013-08Department
Massachusetts Institute of Technology. Department of BiologyJournal
Proteins: Structure, Function, and Bioinformatics
Publisher
Wiley Blackwell
Citation
Andersen, Kasper R., Nina C. Leksa, and Thomas U. Schwartz. “Optimized E. Coli Expression Strain LOBSTR Eliminates Common Contaminants from His-Tag Purification.” Proteins: Structure, Function, and Bioinformatics 81, no. 11 (August 23, 2013): 1857–1861.
Version: Author's final manuscript
ISSN
08873585
1097-0134