A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies
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s43856-021-00045-9.pdf
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Published version
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2.06 MB
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Author(s)
Kongsuphol, Patthara
Jia, Huan
Cheng, Hoi Lok
Gu, Yue
Shunmuganathan, Bhuvaneshwari DO
Chen, Ming Wei
Lim, Sing Mei
Ng, Say Yong
Tambyah, Paul Ananth
Nasir, Haziq
Date Issued
November 11, 2021
Journal
Communications Medicine
Publisher
Springer Science and Business Media LLC
Citation
Kongsuphol, P., Jia, H., Cheng, H.L. et al. A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies. Commun Med 1, 46 (2021).
Version
Final published version
Abstract
Background Neutralizing antibodies (NAbs) prevent pathogens from infecting host cells.
Detection of SARS-CoV-2 NAbs is critical to evaluate herd immunity and monitor vaccine
efficacy against SARS-CoV-2, the virus that causes COVID-19. All currently available NAb
tests are lab-based and time-intensive.
Method We develop a 10 min cellulose pull-down test to detect NAbs against SARS-CoV-2
from human plasma. The test evaluates the ability of antibodies to disrupt ACE2 receptor—
RBD complex formation. The simple, portable, and rapid testing process relies on two key
technologies: (i) the vertical-flow paper-based assay format and (ii) the rapid interaction of
cellulose binding domain to cellulose paper.
Results Here we show the construction of a cellulose-based vertical-flow test. The developed test gives above 80% sensitivity and specificity and up to 93% accuracy as compared
to two current lab-based methods using COVID-19 convalescent plasma.
Conclusions A rapid 10 min cellulose based test has been developed for detection of NAb
against SARS-CoV-2. The test demonstrates comparable performance to the lab-based tests
and can be used at Point-of-Care. Importantly, the approach used for this test can be easily
extended to test RBD variants or to evaluate NAbs against other pathogens.
MIT Department
Singapore-MIT Alliance in Research and Technology (SMART)
Massachusetts Institute of Technology. Department of Chemical Engineering
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DOI of Published Version
https://doi.org/10.1038/s43856-021-00045-9
