Hyper- and hypo- nutrition studies of the hepatic transcriptome and epigenome suggest that PPARα regulates anaerobic glycolysis
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Soltis-2017-Hyper- and hypo- nutrition studies.pdf
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2.71 MB
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Author(s)
Vernia, Santiago
Kennedy, Norman J.
Davis, Roger J.
Soltis, Anthony Robert
Motola, Shmulik
Ng, Christopher W.
Dalin, Simona
Matthews, Bryan
Fraenkel, Ernest
Date Issued
March 2017
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Soltis, Anthony R.; Motola, Shmulik; Vernia, Santiago; Ng, Christopher W.; Kennedy, Norman J.; Dalin, Simona; Matthews, Bryan J.; Davis, Roger J. and Fraenkel, Ernest. “Hyper- and Hypo- Nutrition Studies of the Hepatic Transcriptome and Epigenome Suggest That PPARα Regulates Anaerobic Glycolysis.” Scientific Reports 7, no. 1 (March 2017): 174 © 2017 The Author(s)
Version
Final published version
Abstract
Diet plays a crucial role in shaping human health and disease. Diets promoting obesity and insulin resistance can lead to severe metabolic diseases, while calorie-restricted (CR) diets can improve health and extend lifespan. In this work, we fed mice either a chow diet (CD), a 16 week high-fat diet (HFD), or a CR diet to compare and contrast the effects of these diets on mouse liver biology. We collected transcriptomic and epigenomic datasets from these mice using RNA-Seq and DNase-Seq. We found that both CR and HFD induce extensive transcriptional changes, in some cases altering the same genes in the same direction. We used our epigenomic data to infer transcriptional regulatory proteins bound near these genes that likely influence their expression levels. In particular, we found evidence for critical roles played by PPARα and RXRα. We used ChIP-Seq to profile the binding locations for these factors in HFD and CR livers. We found extensive binding of PPARα near genes involved in glycolysis/gluconeogenesis and uncovered a role for this factor in regulating anaerobic glycolysis. Overall, we generated extensive transcriptional and epigenomic datasets from livers of mice fed these diets and uncovered new functions and gene targets for PPARα.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/s41598-017-00267-9
