Gene expression profiles and transcriptional regulatory pathways underlying mouse tissue macrophage identity and diversity
Name
Regev_Gene expression with figs and supp files.pdf
Size
6.11 MB
Format
Adobe PDF
Checksum (MD5)
31da18af2060e6dc271d5817f967152e
Author(s)
Regev, Aviv
Immunological Genome Project Consortium
Alternative Title
Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages
Date Issued
September 2012
Journal
Nature Immunology
Publisher
Nature Publishing Group
Citation
Gautier, Emmanuel L, Tal Shay, Jennifer Miller, Melanie Greter, Claudia Jakubzick, Stoyan Ivanov, Julie Helft, et al. “Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages.” Nature Immunology 13, no. 11 (September 30, 2012): 1118-1128.
Version
Author's final manuscript
Abstract
We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were not present in all macrophages. Nonetheless, well-characterized surface markers, including MerTK and FcγR1 (CD64), along with a cluster of previously unidentified transcripts, were distinctly and universally associated with mature tissue macrophages. TCEF3, C/EBP-α, Bach1 and CREG-1 were among the transcriptional regulators predicted to regulate these core macrophage-associated genes. The mRNA encoding other transcription factors, such as Gata6, was associated with single macrophage populations. We further identified how these transcripts and the proteins they encode facilitated distinguishing macrophages from dendritic cells.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/ni.2419
